英文名称 Yeast Genomic DNA Extraction Kit 储存条件 酶附件-20℃,其它试剂RT,复检期1年 单位 盒 规格 50T 100T 本试剂盒采用可以特异性结合DNA的离心吸附柱和独特的缓冲液系统,提取酵母基因组DNA。离心吸附柱中采用的硅基质材料为本公司特有新型材料,能够高效专一吸附DNA,可最大限度去除杂质蛋白及细胞中其他有...
Wizard® Genomic DNA Extraction kit (WIZ) Detergent, RNase treatment, NaCl-containing buffer – – Isopropanol. Nucleospin (Clontech) Proteinase K, chaotropic salts – – Binding to a silica membrane – Wizard DNA Clean Up System (Promega) with proteinse K previous digestion Proteinase K, SDS...
This protocol includes the overnight incubation at 50°C in a demineralization/lysis buffer, the phenol-chloroform extraction, the purification of the DNA on silica-filter columns (Cleanmix kit, Talent, Trieste, Italy) and finally the concentration of the samples with Microcon® YM30 (Millipore,...