Long LK (龙良鲲), Yang SZ (羊宋贞), Yao Q (姚青), Zhu HH (朱红惠) (2005). DNA extraction from arbuscular my- corrhizal fungi and analysis by PCR-denaturing gradient gel electrophoresis. Mycosystema (菌物学报), 24, 564- 569. (in Chinese with English abstract)...
PowerSoil® DNA Isolation Kit包含了我们创新专利抑制因子去除技术®(IRT),用于提取所有类型环境样品高质量DNA。环境样品包括堆肥、底泥、粪肥等富含腐植酸的各种难提土样样品。提取出来的高质量DNA可直接进行PCR扩增。PCR扩增结果表明提取到了多种微生物,其中包括细菌(如枯草芽孢杆菌、炭疽杆菌)、真菌(如酵母、霉菌)...
1) DNA gel extraction DNA凝胶回收 2) gel purification 凝胶回收纯化 3) DNA extraction DNA回收 4) DNA recovery DNA回收 5) DNA gel electrophoresis DNA凝胶电泳 例句>> 6) condensate recovery 凝液回收 例句>> 补充资料:Sds-聚丙烯酰胺凝胶电泳凝胶电泳 ...
DNA extraction from AH, blood plasma, primary tumor samples, and cultured cells cfDNAs were extracted from AH or blood plasma using the QIAgen QIAamp Circulating Nucleic Acid kit (Qiagen, Valencia, CA USA) as described by the manufacturer. Formalin-fixed, paraffin embedded (FFPE) tumor sections...
2) Agarose gel electrophoresis DNA琼脂糖凝胶电泳 1. MethodsThe methods of agarose gel electrophoresis,microscope fluorescence of Hoechst 33342 fluorescence dye and flow cells analysis were used in the experiment. 结果经d 儿茶精作用的模型肝细胞DNA琼脂糖凝胶电泳图谱有显著性变化,DNA分子的“梯状”...
pH of lysate and concentration of guanidine hydrochloride on the extraction efficiency of genomic DNA. The optimized method was verified by gel electrophoresis experiments. The results showed that the genomic DNA extraction effect was best and the efficiency was highest when the amount of magnetic bead...
Thermo Scientific NanoDrop instrument was used to evaluate the quality and concentration of the extracted DNA. The amplification length of the COI gene fragment was verified using 1.2% agarose gel electrophoresis and visualizing bands. Finally, high-quality reads were obtained using the mBRAVE platform...
1.3.3琼脂糖凝胶电泳(agarose gel electrophoresis,AGE)试验取扩增产物9 μl,加入PBE缓冲液1 μl及Maker 6 μl同时上样,经3.0%的AGE(100 V,40 min),在紫外凝胶成像系统下观察所得DNA的PCR扩增结果。 2结果 2.1 4种组织所提取DNA的OD260/OD280值及其质量的比较 在DNA的提取过程中,由于甲醛的影响因素,心肌...
By combining agarose gel electrophoresis and extraction, you can easily concentrate dilute, aqueous DNA solutions. After processing with the kit, the DNA can be recovered in a volume of 20 to 50 μL. Isolated DNA fragments are free of inhibitors that could affect common downstream procedures. Fo...
, in duplicate or triplicate. Total DNA was extracted using the CTAB approach5. Total DNA quantity was measured with a Qubit Fluorometer (Thermo Fisher Scientific). Genomic DNA was quality controlled using Nanodrop measures, Qubit quantifications and visualization on a 1% agarose gel electrophoresis....