Nevertheless, non-targeted metagenomic sequencing of semen samples is challenging due to the large number of sperm cells and high amounts of intracellular and extracellular human DNA, which can obscure microbial DNA. In this study, we assessed and refined three methods for DNA extraction from semen...
Application of Maxwell 16 in DNA extraction from old semen samplesGUO Xiao-tao, GUO Jian-wei, DU Yong, LI Yong, CHEN Xue-meiAuthor information + History + 摘要 目的 利用Maxwell 16裂解纯化法从保存8年以上陈旧精斑检材中获取精子DNA。方法 8份陈旧精斑检材采用Maxwell 16裂解纯化法提取精子DNA,并...
DNA extraction from bulk samples Frozen whole blood underwent DNA extraction using the Gentra Puregene Blood Kit (Qiagen). Briefly, 1–2 ml of frozen blood was thawed, lysed in RBC lysis solution and centrifuged. Cell pellet was resuspended in cell lysis solution and incubated at 37 °C ...
4.1 DNA extraction Laboratories performing forensic casework will be properly equipped to extract a wide range of samples from minute bloodstains and tissue fragments, to semen stains and saliva swabs. These laboratories will be able to use their own established extraction protocols, and there is no...
many DNA extraction methods will work equally well for tissues such as liver, but fibrous tissues such as heart, fatty tissues such as brain, and nuclease-rich tissues like spleen present challenges for DNA isolation. We offer a wide range of DNA extraction fr...
many DNA extraction methods will work equally well for tissues such as liver, but fibrous tissues such as heart, fatty tissues such as brain, and nuclease-rich tissues like spleen present challenges for DNA isolation. We offer a wide range of DNA ...
DNA extraction is an important first step in DNA analysis for biomedical and forensic purposes. The DNA extracted from biological samples must be free from contaminants, such as protein and RNA. Although DNA may have to be extracted from different sources for forensic purposes such as saliva, se...
6.4.4 The Process of DNA Extraction As discussed earlier, important DNA evidence sources may be semen, blood, hair, and skin debris. Other sources are also present but are less useful such as urine and feces, as the isolation of genetic material can only be obtained from them in theory bu...
For the semen-spiked buccal swabs, Erase outperformed the standard methods in all five laboratories and in most cases was able to provide a clean male profile from buccal swabs spiked with only 1,500 sperm. The vaginal swabs taken after consensual sex and the evidence collected from rape victim...
DNA profiles obtained from semen stains left at the scenes of crime gave information that one and the same man had committed all the rapes. The Y-chromosome haplotype (9 loci) obtained was used for the elimination process of 421 suspects. One man was found who had an identical DNA profile...