The number of DNA double-strand breaks was estimated by comparing the changes in the rates of 纬-H2AX foci formation in the peripheral blood before and after CT examination. The absorbed dose and effective dose were calculated with the software VirtualDose based on the Monte Carlo method, and ...
2.0 µl primers or probes, 5 units of FastDigest™ HIND III restriction enzyme (Fermentas) per ≤ 1 ng of DNA, and 12.5 µl supermix (EvaGreen dye #1863010, Bio-Rad), with the balance being autoclaved, double distilled water. Assays...
No DNA double strand breaks (DSBs) are induced in buccal mucosal cells (BMCs) after cone beam computed tomography (CBCT) examination, neither in children nor in adults. No significant increases in the amount of γH2AX/53BP1 co-localized foci were observed 30 minutes and 24 hours after ...
Also DNA double-strand breaks may be repaired by an inaccurate repair process leading to mutations. In addition, a double strand break can cause rearrangements of the chromosome structure (possibly disrupting a gene, or causing a gene to come under abnormal regulatory control), and, if such a ...
ANA:antinuclear antibody anti-DNA:anti–double-stranded (native)DNA antibody anti-HIV-1:anti–human immunodeficiency virus 1 antibody anti-HIV-2:anti–human immunodeficiency virus 2 antibody ASO:antistreptolysin O titer DAT:direct anti–human globulin test ...
产品介绍Component of the MRE11/RAD50/NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle ch...
A) Crossing over between two double-stranded DNA molecules (dsDNA) can occur in a region where two different alleles of the same gene have a single-nucleotide change. In the blue strand of DNA the “r” allele has the sequence GGCC. In the green strand of DNA, the “R” allele has ...
reflect the different molecular structures that contain DNA (e.g. mononucleosomes, oligonucleosomes, hemi-nucleosomes, short sized transcription factors binding double strand DNA and so on) that are released from the cells and that undergo dynamic degradation in blood by endonucleases or exonuclease...
DNA double-strand breaks (DSBs) are the most dangerous of such damages. Cells have developed several mechanisms to maintain genomic integrity and repair the damaged DNA. The error-prone classical non-homologous end joining (cNHEJ) and error-proof homologous recombination (HR) are the most ...
In vitro kinase assay indicated that wild-type (WT) cGAS was significantly phosphorylated by DNA-PK, while the phosphorylation of T68E and S213D mutants was greatly reduced (Supplementary Fig.4e). Double mutations of T68E and S213D further decreased cGAS phosphorylation by DNA-PK (Supplementary ...