A frequent cause of concern among investigators performing quantitative RT-PCR is inaccurate data due to DNA contamination in RNA preparations. Although DNA contamination is easily detected by performing a "no-RT" control, there is no easy remedy. In this technical bulletin, we present data showing...
An important problem in measurement of messenger RNA (mRNA) levels by reverse transcription-polymerase chain reaction (RT-PCR) is DNA contamination, which can produce artifactually increased mRNA concentration. Current methods to eliminate contaminating DNA can compromise the integrity of the RNA, are ...
PCR污染清除剂(DNA Contamination Erasol)提供了更简单、更快速和更高质量的方法,包括质粒提取、胶回收、PCR产物纯化、基因组提取、RNA分离纯化、PCR/RT-PCR、荧光定量、克隆载体和感受态细胞制备等数百种实验常用试剂盒。 PCR污染清除剂(DNA Contamination Erasol)产品及特点 ...
人体细胞没有逆转录酶，一般mRNA在细胞质内发挥作用后很快会被降解，不会进入细胞核整合进自身的DNA。但...
5 tips to make your single-cell RNA-seq experiments a success Using UMIs in NGS experiments Web and mobile apps One-step vs. two-step RT-qPCR Avoid DNA contamination in PCR When your his-tagged constructs don't bind Women in STEM That's Good Support! About our blog Contact...
so that their differences in fold DNA removal could be accurately calculated. Using these conditions, the original formulation of TURBO DNA-free reduced gDNA that could be PCR amplified by 451-fold. The improved kit reduced the gDNA contamination much further, by 277,000-fold, or ...
The 1-propynylation at C5 of consecutive pyrimidines in DNA can enhance DNA:RNA hybrid stability at 37 °C by over 1 kcal/mol of substitution [Barnes, T. W., III; Turner, D. H. J. Am. Chem. Soc. 2001, 123, 41074118]. To provide information on the structural consequences of propy...
这种条件培养基通常由重组小鼠细胞系生产，增加了细胞污染的风险。其他常见的培养成分也可能引入污染，如使用小鼠3T3-J2成纤维细胞饲养层和Matrigel基质。发表在Frontiers in cell and developmental biology期刊，题为Low-Level Mouse DNA in Conditioned Medium Generates False Positive Cross-Species Contamination Results ...
It is specific—performed measurement is selective for DNA or RNA It is sensitive—can measure pg/mL; it is the recommended method for very diluted nucleic acid samples It is accurate despite contamination being present in the sample, including nucleic acid contaminants What are the disadvantages?
Because of PCR's exquisite sensitivity, contamination from another DNA source is always a potential problem. Contaminating DNA can come from other samples, or even the operator. The commonest source for contamination is amplified products from previous tests. For the genetic disorders, contamination ...