Presented here is a complete molecular cloning protocol consisting of a number of separate but interconnected methods such as preparation of competent cells; in vitro DNA and ligation; ; DNA gel electrophoresis and gel extraction; and screening transformants by colony , analytical restriction digests,...
So for the typical precipitation protocol, isopropanol is added from between 0.7–1 volumes of sample, and ethanol is added at 2-2.5 volumes of sample. Choosing the Right Solvent: Sample Volume If you are precipitating small volumes of DNA, and you can fit the required amount of solvent ...
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HTS can be advantageous when targeting multiple species via eDNA metabarcoding, making it possible to eliminate the costly and time-consuming steps of cloning and sequencing of single PCR products by Sanger sequencing [3]. A detailed comparison of many sequencing platforms and approaches used for eDN...