A quasi-one-dimensional organic semiconductor, hepta(p-phenylene vinylene) (HPV), was incorporated into a DNA tensegrity triangle motif using a covalent strategy. 3D arrays were self-assembled from an HPV-DNA pseudo-rhombohedron edge by rational design and characterized by X-ray diffraction. ...
Spectroscopy and cryo–electron microscopy showed that for the (8,3)-SWCNT enantiomer, the sequences C3GC7GC3 regularly created an ordered helical structure with a 6.5-angstrom periodicity in which G bridges two carbon atoms. —PDS Abstract Covalent modification of carbon nanotubes is a promising ...
Topoisomerase II binds to DNA and breaks the double strand at the same time. Two Tyr OH forms covalent bonds with 5'-P at the incision. By changing the conformation of the enzyme, the double strand passes through the incision and then re binds. The result is the introduction of negative ...
The immobilization by covalent bonds is the primary method, and the pH, ion strength, type and concentration of DNA probe can affect the effects of immobilization. Keywords:DNA biosensor, DNA, gold surface, immobilization 核酸是重要的生命物质,其保存和传递生物遗传信息的独特方式,即特定的碱基序列和...
Herein, we present a site‐specific photochemical mechanism for the reversible covalent stabilization of stacking bonds in DNA assemblies. To this end, we modified one blunt end with the 3‐cyanovinylcarbazole (cnvK) moiety and positioned a thymine residue (T) at the other blunt end. In the ...
(B) Gyrase binds DNA, transiently breaks both strands of the DNA, and forms covalent bonds with both 5′ ends of the DNA and a tyrosine on the enzyme. (C) Another part of the plasmid passes through the break in the duplex in a “strand passing” reaction. (D) On ligation, there ...
R. et al. Translesion synthesis: Y-family polymerases and the polymerase switch. DNA Repair (Amst) 6, 891–899 (2007). Article CAS Google Scholar Andersen, P. L., Xu, F. & Xiao, W. Eukaryotic DNA damage tolerance and translesion synthesis through covalent modifications of PCNA. Cell ...
However, the high energies associated with many earlier MS techniques which result in fragmentation of covalent bonds, prevent its application for studying weaker intermolecular interactions. The advent of soft ionisation methods such as matrix assisted laser desorption ionisation (MALDI) and electrospray ...
two different DNA molecules into one, since sticky ends contain complementary bases that can be paired.Basepairing of single-stranded ends brings two different DNA molecules together; another key enzyme inrecombinant DNAtechnology calledDNA ligaseestablishescovalent bondsbetween strands of two DNA ...
and covalent linkage of synapsed broken DNA ends11,22, comparatively less is known about the first step, i.e., how the two DSB ends are brought into proximity to achieve synapsis. Fig. 1: A model of DSB synapsis, mediated by DNA loop extrusion....