The DNA sensor cGAS detects cytosolic DNA and instigates type I interferon (IFN) expression. Recent studies find that cGAS also localizes in the nucleus and binds the chromatin. Despite the mechanism controlling nuclear cGAS activation is well elucidated
No DNA double strand breaks (DSBs) are induced in buccal mucosal cells (BMCs) after cone beam computed tomography (CBCT) examination, neither in children nor in adults. No significant increases in the amount of γH2AX/53BP1 co-localized foci were observed 30 minutes and 24 hours after ...
Cell Type-Specific Quantification of Telomere Length and DNA Double-strand Breaks in Individual Lung Cells by Fluorescence In Situ Hybridization and Fluorescent Immunohistochemistry Authors:van Batenburg, A. A., Kazemier, K. M., Peeters, T., van Oosterhout, M. F. M., van der Vis, J. J.,...
上海钰博生物科技有限公司是2012年5月由“海归”组成的创业团队与两个专业投资机构发起成立的,以开发生产科研试剂和特色检验产品为方向,经过两年多的努力,已经建立了免疫学和分子生物学的五个技术平台,开发了ELISA/ELISpot酶联免疫试剂盒、重组蛋白、荧光标记抗体、四聚体(Tetramer)特异性T细胞检测试剂盒、Aimplex流式高...
Cell Biolabs——OxiSelect™ DNA Double Strand Break Assay(DNA双链断裂分析试剂盒)(货号:STA-321); abcom——人H2A.X (phospho S139) In-Cell ELISA试剂盒(IR) (ab131382); CST——Phospho-Histone H2A.X (Ser139) Antibody #2577; 03\TUNEL检测—基于DNA损伤后形成的产物检测DNA损伤 ...
The presence of the complementary oligonucleotide allows a second strand of complementary DNA to be synthesized. This constitutes one cycle of PCR. When this cycle is repeated, the segment of DNA in between the oligonucleotides is geometrically increased. RNA can also be amplified by first ...
DNA双链断裂(double-strand breaks,DSBs)是细胞内最严重的DNA损伤形式之一。同源重组修复和非同源末端链接是修复DSBs的两种重要途径。目前,在绝大多数古菌中只发现了同源重组修复途径,对参与该途径的保守蛋白Mrell,Rad50和重组酶RadA(真核生物中为Rad51)都有深入的研究。
Further investigation is needed to determine whether it originates from double-strand breaks, breakage-fusion-bridge, or replication slippage in the SORBS1 gene of the liver or other tissues with insulin resistance. In addition, we could not deduce whether the increased intracellular content of ...
DNA damage signaling;double-strand breaks repair;DNA repair inhibitor;AsiDNA 1. Introduction DNA damage can have deleterious effects, as it interferes with DNA replication and transcription, and it can ultimately result in mutations and chromosomal aberrations, and cell death in highly proliferating cel...
The genomes of badnaviruses are 7.2 to 9.2 kb long relaxed circular DNA double strands with single-strand overlaps. Those are marks of the starts of plus- and minus-strand DNA synthesis by reverse transcription [23]. Reverse transcription occurs within the virus capsid and the resulting DNA ...