他们之所以能提出双螺旋结构,是因为他们发现,在自然界中腺嘌呤(A)是与胸腺嘧啶(T)配对的,鸟嘌呤(G)是与胞嘧啶(C)配对的。正是这种稳定可靠的碱基互补配对原则,让双螺旋结构有了根基。 不过,就在沃森和克里克领走诺奖没多久,一些...
A. 正常的双链DNA;B. 顺铂(cisplatin);C. 补骨脂素/扫若仑(psoralen);D. 亚硝基脲(BCNU/nitrosourea);E. 乙醛(酒精代谢的中间产物)。 下图:甲醛(或其他低级醛类)所可能造成的DNA双链交联损伤的化学形式[3],其中的几种——包括碱基与互补链的(脱氧)核糖-磷酸骨架所发生的交联(下图(7),“dA-AP”),已经...
论文摘要 The B-DNA double helix can dynamically accommodateG-C and A-T base pairs in either Watson–Crick or Hoogsteen configurations.Here, we show that G-C+(in which + indicates protonation) and A-UHoogsteen base...
Because in order to be stable in cells, the two strands of a DNA double helix have to form base pairs. 因为为了保持细胞的稳定,DNA双螺旋结构中的两条链必须形成碱基对。 And because C only pairs with G, and T only pairs with A, simply changing a C to a T on one DNA strand creates ...
(延伸能力可达约7kb);RuvC是一能特异性的识别和结合Holliday连接体的核酸内切酶(RuvC对Holliday连接体的亲和力比对dsDNA的亲和力高103-104倍),包含两个19kDa的亚基,可在ruvAB的协助下(通过Holliday连接体的分支迁移)找到5'-(A/T)TT↓(G/C)-3'序列(RuvC的作用热点,平均每64bp出现一次),从而对Holliday连接体...
第四章DNA的突变、损伤和修复 第一节：突变（mutation)一、突变的主要类型 突变是DNA碱基序列水平上的永久的、可遗传的改变。碱基序列的变化可以分为下面几种类型：碱基替换（basesubstitution），即DNA分子中一个碱基被另一个碱基替代；插入（insertion），涉及一个或多个碱基插入到DNA序列中；缺失（deletion），涉及...
The synthetic deoxyoctanucleotide d(G-G-G-G-T-C-C-C) crystallizes as an A-type DNA double helix containing two adjacent G · T base-pair mismatches. The structure has been refined to an R-factor of 14% at 2.1 resolution with 104 solvent molecules located. The two G · T mismatches...
To test whether TC-DPC repair of actively transcribed genes was a genome-wide response, we divided the DPC sequencing (DPC-seq) results in bins of 1 kb and analysed DPC repair in expressed bins (>3 transcripts per million base pairs (TPM)). This analysis showed that ~50% of all DPCs...
DNA is well-documented to stimulate immune response. However, the nature of the DNA to activate immune surveillance is less understood. Here, we show that the activation of cyclic GMP-AMP synthase (cGAS) depends on DNA mechanical flexibility, which is co
C. zofingiensis Whole genome 15,274 predicted protein-coding genes. Two genes encoding beta-ketolase, a key enzyme for astaxanthin synthesis, and candidate genes that may be involved in astaxanthin biosynthesis. (Roth et al., 2017) 3.1.2 Functional genomics The primary goal of functional genom...