and-paste DNA insertion with up to 99.4% simple insertion product purity, while retaining robust integration efficiencies on genomic targets. HELIX has substantially higher on-target specificity than canonical CASTs, and we identify several novel factors that further regulate targeted and genome-wide ...
By leveraging distinct features of both type V-K and type I systems, HELIX enables cut-and-paste DNA insertion with up to 99.3% simple insertion product purity, while retaining robust integration efficiencies on genomic targets. Furthermore, we demonstrate the versatility of this approach by ...
Chapter 5 - Some Basic Tools: How to Cut, Paste, Copy, Measure, Visualize, and Clone DNA Chapter 5 - Some Basic Tools: How to Cut, Paste, Copy, Measure, Visualize, and Clone DNAdoi:10.1016/B978-0-12-415874-0.00005-6DNA extraction... MA Hoy - Elsevier Inc. 被引量: 0发表: 2013...