DMSO concentration of 30 v/v%, however, had a cytotoxic effect on cell viability. Thus, 20 v/v% DMSO concentration was found to be the most suitable for cryopreservation of HepG2 cells in the adhesion state.Hiroaki SEKINEMari OTAKI
cell morphology was observed under microscopy,cell viability and cellular apoptosis were tested by a colorimetric assay with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT) and flow cytometry respectively.Results When the DMSO concentration was lower than 2%,PC12 cells were able...
How much DMSO is present in Product C6164, Cell Freezing Medium-DMSO? 1 answer Technical Support · 2 years ago The DMSO is at a 10% concentration. Helpful? Yes · 0 No · 1 Report Anonymous · 2 years ago What is the Department of Transportation shipping information for this prod...
(0, 0.125, 0.25, 0.5, 1.0, and 2.0 μg/mL) are each inoculated with a 100 μL suspension of C. albicans in late log phase to yield a final cell concentration of 10 6 /mL. The tubes are aerobically incubated at 35°C, and at each time point (0, 1, 2, 4, 6, 12, and 24...
Concentration / Solvent Volume / Mass1 mg5 mg10 mg 1 mM2.1325 mL10.6623 mL21.3247 mL 5 mM0.4265 mL2.1325 mL4.2649 mL 10 mM0.2132 mL1.0662 mL2.1325 mL 不同实验动物依据体表面积的等效剂量转换表(参考来源于公开文献) 小鼠大鼠兔豚鼠仓鼠狗
The laurdan powder was dissolved in DMSO (9 mM final), and the solution was diluted in RPMI 1640 medium (33 µM final concentration). Cells in the medium were incubated for 30 min at ambient temperature. Fluorescence images of cells were captured by a confocal microscope (TCS-SP8, Leica...
Cell TypeHepatocyte Cells Concentration10 mM FormLiquid Product TypeDMSO Shelf Life13 Months from date of manufacture Without AdditivesNo Phenol Red For Use With (Application)Cell Culture Quantity50 μL Unit SizeEach Contents & Storage Store in refrigerator (2 to 8°C) and protect from light浏览...
Reducing the concentration of DMSO to 1% allows immediate reversal of the condition. Paramecium polyribosomes subjected to 4% DMSO either in whole cell homogenates or during purification through sucrose density gradients appear unaffected while cycloheximide at concentrations up to 100 渭g/ml did not ...
Measurement of glucose uptake and intracellular calcium concentration in single, living pancreatic beta-cells. Authors:Yamada K, Nakata M, Horimoto N, Saito M, Matsuoka H, Inagaki N Journal:J Biol Chem PubMed ID:10748091 'There has been no method previously to measure both glucose transport and...
concentration of electrolytes, pH changes, and denaturation of proteins. These lethal effects are minimized by adding a cryoprotective agent which lowers the freezing point and allows for a slower cooling rate. BPC-tested DMSO also meets the requirements of biotechnology and tissue engineering applicati...