总之,本研究鉴定了一个有助于SCC化疗耐药的关键非编码RNA DLGAP1-AS2(D-AS2),其在化疗耐药SCC细胞中升高并与代谢事件相关。研究结果表明DLGAP1-AS2介导的磷脂酸合成激活YAP信号通路并赋予鳞状细胞癌的化疗耐药。 原文链接: https://aacrjournals.org/cancerres/article-abstract/doi/10.1158/0008-5472.CAN...
siRNA-DLGAP1-AS2 转染优化:经过实验,确定了 siRNA-DLGAP1-AS2 转染 A549 细胞的最佳浓度为 20 pmol,最佳时间为 48 h,转染效率高达 99.6%。 联合治疗增强细胞毒性:MTT 检测结果表明,降低 DLGAP1-AS2 的表达能够增加 A549 细胞对卡铂的敏感性,联合治疗的协同系数(CDI)为 0.88,具有协同效应。联合治疗显著降低了...
为了进一步阐明D-AS2在体内的功能,作者将对照或D-AS2过表达的UM1细胞皮下移植到裸鼠体内,并在一周后给予生理盐水或DDP处理。与体外实验结果一致,D-AS2过表达UM1细胞显示出较低的生长速率,并对DDP抗药性增加(Fig. 2C),而在亲本KYSE...
研究者发现DLGAP1-AS2通过与Elongin A(Elongin A,Elongin A)的物理相互作用促进肿瘤的发生和转移,并通过促进含有21(Trim21)的三个基序(Trim21)介导的泛素化修饰和ELOA的降解来抑制其蛋白质稳定性,从而促进肿瘤的发生和转移。 特别是,研究者发现DLGAP1-AS2通过抑制ELOA介导的LHPP的转录激活,从而阻断LHPP依赖的AKT信号...
Functionally, DLGAP1-AS2 overexpression promoted the aerobic glycolysis and DLGAP1-AS2 knockdown suppressed the tumor growth of NSCLC cells. Mechanistically, m6A methyltransferase METTL3 enhanced the stability of DLGAP1-AS2 via m6A site binding. Moreover, DLGAP1-AS2 interacted with YTHDF1 to enhance...
DLGAP1-AS2miR-503Cyclin D1Background LncRNA DLGAP1-AS2 plays an oncogenic role in glioma, while its role in other cancers is unknown. This study aimed to study the role of DLGAP1-AS2 in non-small cell lung cancer (NSCLC). Methods Expression of DLGAP1-AS2 in NSCLC and paired non-...
However, DLGAP1-AS2 overexpression accelerated the progression of OS. We further found that DLGAP1-AS2 upregulation was induced by hypoxia and low glucose. Additionally, DLGAP1-AS2 bound to miR-451a to upregulate HK2 expression. Rescue assays revealed that the DLGAP1-AS2/miR-451a/HK2...
The long noncoding RNA (lncRNA) DLGAP1-AS2 has recently been characterized as an oncogenic lncRNA in several cancers. However, its biological roles and clinical significance in gastric cancer (GC) remains barely understood. In this study, we performed a systematic analysis of DLGAP1-AS2 ...
LncRNA-DLGAP1-AS2Despite being one of the primary and most effective treatments for advanced stages of lung cancer, chemotherapy drugs like carboplatin have limitations due to their adverse side effects and the development of drug resistance in lung cancer cells. However, recent studies have shown ...
Methods:HR8348-R cells, radioresistant cells from HR8348 after irradiation, were isolated into CD133 negative (CD133 Results:The DLGAP1-AS2 level was increased in CD133 Conclusion:DLGAP1-AS2 accelerates the radioresistance of rectal cancer cells through interactions with E2F1 to upregulate CD151 ...