each containing single cells; and (2) detects and quantifies in real time the production of lactate or hydrogen ions by single cells, by employing a setup that utilizes an inverted microscope to interrogate droplets that flow into a microfluidic channel, by a laser-induced fluorescence [96]. ...
Multiple fluorophores can be applied on a single tissue section and are interrogated using a multi-spectral microscope [41,58]. This technology enables a comprehensive characterization of the topography and spatial relationship Biomedicines 2018, 6, 110 6 of 24 between tumor cells and stromally ...