The most conserved residue position was H29, for which the aromatic/hydrophobic side chain is expected to stabilize the type 1 canonical structure of the CDR-H1 loop by packing with the upper hydrophobic core of the VH domain16. The other conserved residue position is H32, where the aromatic...
After the incubation, the medium was removed, cells were washed with PBS 1×, and the cells were fixed with Carnoy’s solution (1:3 methanol: acetic acid) (J. T. Baker) and stained with crystal violet (0.5 % m/v) for 10 min. The number of established colonies (50 or more cells/...
Pandemic and epidemic outbreaks of influenza A virus (IAV) infection pose severe challenges to human society. Passive immunotherapy with recombinant neutralizing antibodies can potentially mitigate the threats of IAV infection. With a high throughput neutralizing antibody discovery platform, we produced artif...
Such a cascade for the oxidation of methanol has been presented before [35]. However, electrochemical activity and/or performance in a biofuel cell was not demonstrated. Here, we have employed cellulosome machinery and terminology and generated a redox enzymatic cascade to form what we have termed...