As we had more particles in this dataset, we both applied particle subtraction to improve the map quality and also applied local refinement on C34 symmetry-expanded particles from three masked staves. This improved the resolution to 5.9 Å. We used RELION image handler to form a 34-mer C...
a, A model for the flagellar motor from Gram-negative bacteria was built from our structure and that of theS. entericaflagellar basal body (7CGOref.12). The L-ring (light purple) contains FlgH subunits. The P-ring (dark purple) contains the FlgI subunits. In the centre of the LP-rin...