Biotechnology approaches could accelerate the breeding process by introducing critical genes; however, a screening approach will likely be needed to improve the many unknown traits involved in the evolution ofC4plants.Charting New Pathways To C4Rice...
P. C. Directed evolution of proteins by exon shuffling. Nat. Biotechnol. 19, 423–428 (2001). Article CAS PubMed Google Scholar Horton, R. M., Hunt, H. D., Ho, S. N., Pullen, J. K. & Pease, L. R. Engineering hybrid genes without the use of restriction enzymes: gene-...
N.M.G. conceived and directed the work, conducted evolution and Hek293T experiments, performed analyses, and wrote the manuscript. D.K.L. and N.M.S.-E. conducted mammalian cell transfections. H.A.R. conducted all off-target studies and analyses except for whole transcriptome sequencing and...
In monocots, ODM was used to target AHAS in maize (Zhu et al.1999) and rice (Okuzaki and Toriyama2004). In maize, nucleotide changes were induced at two sites, S621A (AGT to AAT) for imidazolinone and sulfonylurea herbicide resistance and P165A, mimicking the point mutation in tobacco i...
Until recently, the CRISPR/Cas-based miRNA editing has only been utilized in plant speciesArabidopsis thaliana[80],Marchantia polymorpha[81], rice [80], wheat [82], soybean [83], and tomato [84]. The efforts to knockoutMIRgenes has been restricted to agronomically important trains, including...
It is possible that those organisms retaining a RNAP II transcription cycle mediated by CDK7 and 8 kinase activity form distinct clades, in both RPB1 and kinase derived trees, because both sets of proteins share parallel modes of evolution driven by their physical interactions. In this case, ...
This bioinformatic analysis of transcriptomes across the plant kingdom reveals stepwise evolution of Pol IV- and Pol V-specific subunits. Article CAS PubMed Google Scholar Ream, T. S. et al. Subunit compositions of the RNA-silencing enzymes Pol IV and Pol V reveal their origins as ...
Protein engineering through machine-learning-guided directed evolution enables the optimization of protein functions. Machine-learning approaches predict how sequence maps to function in a data-driven manner without requiring a detailed model of the underlying physics or biological pathways. Such methods acc...
Lam KC, Ibrahim RK, Behdad B, Dayanandan D: Structure, function, and evolution of plant O-methyltransferases. Genome. 2007, 50: 1001-1013. 10.1139/G07-077. Article PubMed CAS Google Scholar Ferrer JL, Austin MB, Stewart C, Noel JP: Structure and function of enzymes involved in the bi...
Xu, L. et al., “Directed Evolution of High-Affinity Antibody Mimics Using mRNA Display,” Chemistry & Biology, 9(8): 933-942 (Aug. 2002), XP027353363. Primary Examiner: REDDIG, PETER J Attorney, Agent or Firm: HAMILTON, BROOK, SMITH & REYNOLDS, P.C. (LINCOLN, MASSACHUSETTS, US) ...