We confirmed DLK1 expression in the human SCLC cell line Lu-135 (JCRB0170; JCRB cell bank, Osaka, Japan) and the human neuroblastoma cell line SK-N-F1 (ATCC CRL-2142; ATCC, Tokyo, Japan) by IHC using the anti-DLK1 antibody as described above. 2.5. Humanized anti-DLK1 antibody and 125...
HeLa CD4+ cells (154, NIH AIDS reagent program) were maintained in DMEM supplemented with 10% (v/v) FBS and 2 mg/mL G418 (16512-81, Nacalai Tesque, Kyoto, Japan). MT4 cells (JCRB1216, Japanese Collection of Research Bioresources Cell Bank), C8166-CCR5 cells, and THP-1 cells were ...
Human HIP1R (Huntingtin Interacting Protein 1 Related) (GenBank: NM_003959.3; 3,204 bp ORF sequence) in pcDNA3.1+/C-(K)-DYK was purchased from GenScript (NJ, USA). For constructing the point mutants of the putative Pin1 binding serine929-proline site, Ser929 was substituted by alanine...
Human rheumatoid arthritis fibroblast-like synoviocytes (MH7A) were derived from the Riken cell bank in Japan (RCB1512). Small interfering RNA (si-VIM) and overexpression plasmid were purchased from Suzhou Gemma Genetics Co. Annexin V/PI apoptosis kit and NO detection kit were purchased from Bi...
Asians of Tokyo, Japan 90 0.956 0.044 0.0 0.978 0.022 Chinese in Metropolitan Denver, Colorado 168 0.917 0.083 0.0 0.958 0.042 Other ethnic populations or groups Residents of los Angeles, with Mexican ancestry 100 0.940 0.060 0.0 0.970 0.030 Hispanics, cell bank Coriel Cell Repository 44 1.0 0.0...
Nalm 20 cells (pro-B cell line), HPB-NULL cells (pre-B cell line), Ramos cells (Burkitt's-derived B cell line), ER cells (EB-virus transformed B cell line), and IM-9 cells (IgG-secreting myeloma) were obtained from the Japanese Cancer Research Resources Bank and were cultured in ...
Amino acid mutation analysis of Xiamen RSV A and B F protein was performed in comparison with the two reference sequences, RSV A2 strain (GenBank accession no. KT992094) and RSV 9320 strain (GenBank accession no. AY353550). Out of 549 amino acid residues in the F protein without the ...
The sequences were compared with those available sequences in the GenBank database using Basic Local Alignment Search Tool (BLAST) (https://blast.ncbi.nlm.nih.gov/Blast.cgi). In addition, the same set of stool samples was also screened for group A rotavirus (RVA), norovirus (NoV), and...
Briefly, human codon-optimized cDNA-encoding SARS-CoV-2 S glycoprotein (GenBank accession number NC_045512) was synthesized by GenScript and cloned into the eukaryotic cell expression vector pcDNA3.1 between the BamH I and XhoI sites. Pseudovirions were produced by co-transfection of Lenti-X ...
Horseradish peroxidase (HRP) conjugated goat anti-rabbit immunoglobulin G (IgG) antibody was purchased from Medical & Biological Laboratories (Nagoya, Japan). For quantitative polymerase chain reaction (PCR), primers for detection of expression levels of nitric oxide synthase 2 (Nos2) (GenBank ...