, Presented at the Third International Conference on Recirculating Aquaculture, Virginia Polytechnic Institute and State University, Roanoke, VA, 22–23 July 2000, pp. 222–226. Google Scholar Watten et al., 1991 Watten, B.J., Colt, J.E., Boyd, C.E., 1991. Modeling the effect of ...
and motivations of a suspect or victim. One such incident occurred in August 2015 in Roanoke, VA, when former disgruntled WDBJ reporter Vester Flanagan, known on-air as Bryce Williams, shot and killed WDBJ journalists Adam Parker and Alison Ward, and wounded another (the interviewee) while doin...
STZ was slowly injected in the ventricle using a Hamilton microsyringe that was connected to the 33-gauge injection cannula (Plastics One, Roanoke, VA, USA) over 5 min. The needle was left in place for an additional 3 min to allow for diffusion. The vehicle group received 4 μl of ...
Dummy cannulae (C232DC, Plastics One, Roanoke, VA) were inserted into all guide cannulae to maintain patency. Cannulae were fixed to the skull with dental cement (Darby Dental, Jericho, NY) that served to close the wound. 2.3. Drugs and infusions Infusions were performed by gently ...
A stainless steel bipolar recording electrode (0.01 in. diameter, PlasticsOne, Roanoke, VA) was stereotaxically placed into the left dorsal hippocampus (coordinates from bregma: −2.0 mm AP, −1.6 mm ML, and 1.0 mm below dura). A ground wire was fastened to a stainless steel screw (...
For behavioral testing, all rats were implanted with a guide cannula (26 gauge cannula; Plastics One, Roanoke, VA) allowing brain drug injections targeting the lateral ventricle (LV) or DR under ketamine/xylazine anesthesia. The cannulas were positioned and attached to the skull with dental acryl...
Cannulae for striatal infusions were obtained from Plastics One (Roanoke, VA). The D1/D2 dopamine receptor antagonist flupenthixol (Sigma) was infused bilaterally into the dorsal striatum at corrdinates AP = 1.5 mm, ML = ± 1.5 mm DV = −2.5 at 15 μg/0.5 μL/hemisphere. The drug...
All animals were implanted with cannula (Plastics One, Roanoke, VA) aimed at the lateral ventricle two days after baseline testing. The osmotic pump (Alzet, Cupertino, CA: Model 2002), which was implanted S.C. in the intrascapular region, was connected to the cannula by 2 cm of polyethyle...
The guide cannula (Alzet Brain infusion kit III 1–3 mm, Durect Corporation, Cupertino, CA) was secured with screws and cranioplastic cement (Cranioplastic powder, Plastic One Inc., Roanoke, VA; Dentsply repair material, Dentsply International, York, PA). Approximately 40 hr prior to the ...
(−0.4 to −0.6 mm AP; 1.0 mm ML; −4.9 to −5.25 mm DV) and HSV-EF1α-mCherry-IRES-Cre into the VTA (−3.1 to −3.5 mm AP; 0.65 mm ML; −4.3 to −4.6 mm DV) and implanted a cannula (PlasticsOne, Roanoke, VA, USA) above the VTA (−3.3 to −3.5 mm ...