The ability to create gene knockout mice was made possible by the discovery ofpluripotent embryonic stem(ES)cells.These cells are different from hematopoietic pluripotent stem cells in that they have the potential to give rise to not only cells of the blood and immune system, but toanycell type...
Knockout mice have been a useful tool for studying the mechanisms of opacification in the lens and also provided models for testing treatments. However, these animals are often used to study the role of a specific protein in maintaining lens homeostasis, rather than as a model for study catarac...
Nedd8 activating enzyme inhibitor MLN4924, and by VHL knockout, consistent with the mechanism-of-action of a VHL-recruiting degrader (Fig.2band Supplementary Fig.2b). We also observed the expected collapse in cellular signaling and diminished cell proliferation in 2D-monolayer and 3D-spheroid cult...
(LightCycler 480 SYBR Green I Master, Roche Life Science); third, measuring TRPML1 channel activity via the endolysosomal patch clamp technique. The strategy insured the genetic knockout of Exon 2 of human MCOLN1, its first transmembrane domain, the lysosomal targeting sequence (LTS), and a ...
Overlap functions in correlation methods and quasifree nucleon knockout from O-16 - art. no. 014306 The cross sections of the (e,e'N) and (gamma,p) reactions on O-16 are calculated, for the transitions to the 1/2- ground state and the first 3/2(-) excited... G Mk.,A An.,...
that ERα in the efferent ductules is necessary fornormal reabsorption of rete testis fluid in the male tract. In that study, the ERα knockout mouse showed luminal dilation in efferent ductules, rete testis, and seminiferous tubules, suggestive of fluid accumulation. Dilation of the rete testis...
It has been difficult to reproduce experimentally, except for the pig, until recent studies using a liquid alcohol-containing diet in a metallothionen (MT) knockout mouse. MT is a zinc regulatory protein with release of zinc under oxidative stress. The addition of zinc to the diet preve...
Effects of PTC-209 pre-treatment on Bmi1 expression last throughout the reprogramming Considering that 24 h pre-treatment with PTC-209 was sufficient to enhance the efficiency of CiDCR, we investigated the persistence of PTC-209 effects beyond the time of compound administration, throughout the...
point. Significance levels of the pairwise comparisons is indicated as: *p-value < 0.05, **p-value < 0.01, ***p-value < 0.001. cOuter nuclear layer degeneration inMsi1−/−/Msi2−/−knockout retinas. Representative H&E-stained sections from theMsi1+/+/Msi2+/+...
L-779976, a selective somatostatin analog specific to SST2, not only inhibited secretin-stimulated ductal fluid secretion, but also directly induced ductal fluid absorption; in SST2-knockout mice, these effects of somatostatin were diminished.227Thus, somatostatin may regulate ductal bile formation by...