and tips on troubleshooting and avoiding known pitfalls.\nAuthoritative and thorough, __Digital PCR: Methods and Protocols__ is a valuable resource for specialists in various fields, including geneticists, neurologists, immunologists, oncologists, and researchers who are interested in environmental scienc...
Digital PCR can be used to perform rare allele detection, low-fold gene expression determination, viral titering, absolute quantitation of next-generation sequencing libraries, pathogen detection, generation of reference standards, GMO monitori...
Digital PCR (dPCR) quantifies the absolute number of copies of a target genomic or complementary DNA (cDNA) sequence from a sample and can be used for a variety of applications[1–3]. Forgenotypingapplications, dPCR can capture rare allele or copy number variation, be used for liquid biop...
Both quantitative PCR (qPCR) and digital PCR (dPCR) provide sensitive and specific detection, and precise quantification of nucleic acids. Both technologies have similarities, but they have differences that make one or the other the more adapted choice for specific applications....
QuantStudio Absolute Q Digital PCR System Powerfully simple digital PCR Using consumable plates with proprietary microfluidic array plate technology, the Applied Biosystems™ QuantStudio™ Absolute Q™ Digital PCR System allows for over 95% of the sample to be analyzed across over 20,000 ...
A digital PCR-based protocol to detect and quantify RNA editing events at hotspots. Authors:Oh S,Buisson R Journal:STAR protocols PubMed ID:35284835 APOBEC3A, CRISPR programmable RNA base editors, or other enzymes can edit RNA transcripts at specific locations or hotspots. Precise quantification of...
The standard tool to quantify HIV DNA and cell- associated viral RNA has been real-time PCR (qPCR). However, digital PCR has become a promising quanti- fication strategy that combines absolute quantification with high sensitivity [2]. Digital PCR is based on the concept of limiting dilution ...
105 Droplet digital polymerase chain reaction (ddPCR) is a third generation of polymerase chain reaction (PCR) that enables the exact quantification of nucleic acid targets within a sample. From: Progress in Molecular Biology and Translational Science, 2022 ...
PCR in wastewater research Interviewer: Going into the methods a little bit more you mentioned, you know, differences of qPCR, and digital PCR. Did you utilize digital PCR? And what did that look like for your research? Dr. Sarah Philo: We didn’t get a digital PCR...
Digital PCR is accomplished by capturing or isolating each individual nucleic acid molecule present in a sample within many chambers, zones, or regions that are able to localize and concentrate the amplification product to detectable levels.