Labeling DNA by PCR holds advantages over other methods in that the yield of probe is very high, and only a small amount of template DNA is required and the purity of the template DNA is not as crucial as e.g. in random primed labeling....
5) digoxigenin-labeled nucleic acid probe 地高辛标记核酸探针 1. Establishment and application of digoxigenin-labeled nucleic acid probe for PCV-2; 根据GenBank中已发表的猪圆环病毒2型(PCV-2)基因序列,在ORF2内设计1对特异性引物,利用PCR反应扩增出371 bp的核酸片段,回收并纯化PCR产物,用地高辛标记,...
The accessibility of membrane-bound DNA of all of the tested bacteria to the NPRC probe was confirmed by their hybridization to the universal probe EUB 338 (1), with 16S ribosomal DNA as the target...Rost A-K (1995) Nonradioactive Northern blot hybridization with DIG-labeled DNA probes. ...
a当你前后左右都没有路时,一定是命运鼓励你向上飞了 When you all around does not have the road, certainly was the destiny encourages you to fly upwardly[translate] aprobe labeled with DIG_dUTP, indicating that the DNA 探针标记与DIG_dUTP,表明脱氧核糖核酸[translate]...
In standard hybridization solution,IL-1 β gene ss DNA was labeled for DIG. Through the dark color,hybridization band deep purple was displayed in the Hybond N+,which proved that the probe concentration reached the standard concentration 展开 年份: 2015 ...
After hybridization to the embryos, each bound antisense probe is then visualized using a specific enzyme-conjugated antibody and a distinctive color reaction. These procedures work well in situations when both of the target genes are expressed at high levels but are less reliable for cases in ...
Human chromosome painting probes , DIG-labeledCat, Probe
Subventral gland localization of MiPFN3 DIG-labeled cDNA probe in M. incognita J2.Leelarasamee, NatthanonZhang, LeiGleason, Cynthia
- 1990 () Citation Context ...lled pcpB gene probe was synthesized by PCR from genomic DNA of ATCC 39723 using a DIG labelling nucleotide mixture (Boehringer Mannheim Canada, Laval, Que艁bec, Canada) as described by Liesack et al. =-=[16]-=-. The primer pair, PCPB1 (5PCGC GCT ...
After hybridization to the embryos, each bound antisense probe is then visualized using a specific enzyme-conjugated antibody and a distinctive color reaction. These procedures work well in situations when both of the target genes are expressed at high levels but are less reliable for cases in ...