To test whether the up- and down-regulated sequences identified in our mRNAseq study provided valid protein targets to reverse fibrosis, we assessed the ability of more than 30 anti-integrin blocking antibodies to promote or inhibit myofibroblast differentiation. Myofibroblasts are defined by their mo...
We acquired 36 continual slices of functional images per volume (FOV = 208 × 208 mm; 64 × 64 matrix; slice thickness = 4 mm) using a four-segment echo planar imaging (EPI) pulse sequence (echo time [TE] = 20 milliseconds; flip angle...
Design: We examined HF volumetrics on magnetic resonance imaging scans that were acquired with a 1-T magnetic resonance imaging scanner (Siemens Magnetom, Siemens Medical Systems, Iselin, NJ) by using magnetization-prepared rapid gradient echo threedimensional sequences (producing a gapless series of...
mainly including asymmetric synthesis, biological methods and chromatographic methods. Asymmetric synthesis is an ideal method to obtain chiral drugs. This method uses an asymmetric catalyst to induce the reaction, and then undergoes a series of subsequent treatments...
Virus-specific RNA was amplified after reverse transcription using the EURORealTime SARS-CoV-2/Influenza A/B PCR test (EUROIMMUN) allowing simultaneous detection of two target sequences in the SARS-CoV-2 ORF1ab and N genes as well as one target sequence each for influenza virus A and B. ...
sequences from 45 species used, plus an additional three outgroups (Cardiocrinum giganteum,Nomocharis pardanthina, andFritillaia karelinii). This resulted in the amplification of 472 different sequences. The sequencing success rate oftrnF-ndhJ(93.7%) andITS(91.6%) were relatively low, while the...
Product sizes, annealing temperatures and primer sequences are listed in Table 1. RT-PCR products were analyzed by 2.5% agarose ethidium bromide gel electrophoresis. Images were captured with Polaroid film (Hertfordshire, UK) under UV light. Amplification of the housekeeping gene GAPDH transcripts ...
The electrogram sequence upon cessation of the VOP (an atrial–ventricular or atrial–atrial–ventricular response) is useful for diagnosing atrial tachycardias (ATs) (1). In addition, several criteria obtained from VOP provide important clues to distinguish orthodromic reciprocating tachycardia (ORT) ...
All cloning procedures followed protocols described previously34the Invitrogen Gateway Technology Manual, and the Tol2kit wiki (http://tol2kit.genetics.utah.edu/index.php/Main_Page). We cloned thekrt4promoter sequence into p5E (gift from David Grunwald, University of Utah, Salt Lake City, UT, ...
The AUC for the & 2011 Cancer Research UK British Journal of Cancer (2011) 105(9), 1370 – 1378 1374 Table 3 LRG1 and IL-6 in biliary tract cancer NS Sandanayake et al Identification of putative biomarkers from 2D-DIGE discovery experiment Protein name IPI accession Mascot Sequence No. ...