Mice were kept in a pathogen-free environment with a controlled light cycle from 6:00 h to 18:00 h, a temperature of 20 to 23 °C and humidity of 50 to 60%. Mice were allowed to acclimatize for a minimum of 7 days before each experiment....
Sections were viewed in light-field using a Zeiss microscope (Axioskope 2 plus) and captured with an AxioCam HRC (Zeiss) using Axiovision software. For immunofluorescent co-staining, PCNA and GFP antibodies were used at 1:200 and 1:500, respectively in PBST containing 5% goat serum. After ...
Three, 10-s scans were carried out at three points along a 3-µm linear area in both sound and eroded primary enamel, and Raman spectra were obtained with the LabRam 300 fitted with an Olympus BX40 microscope. Scanning electron microscopy was used to confirm the presence of sound and ...
Cells (1×106 cells/ml) were washed in PBS (Beit-Ha’Emek) containing 5% fetal calf serum (Beit-Ha’Emek) at 1250 rpm, 4°C for 10 minutes and fixed with 4% paraformaldehyde (Electron Microscope Sciences, Belgar) at 37 °C for 10 minutes. After chilling in ice for 1 minute cells...
The samples were then dehydrated through a graded series of ethanol, critical point dried and coated with palladium-gold. The coated specimens were examined under a JSM-6301F scanning electron microscope (Joel, Tokyo, Japan). Transmission electron microscopy The treated and untreated HBPCs were ...
Zhang M, Moller M, Broman J, Sukiasyan N, Wienecke J, Hultborn H: Expression of calcium channel CaV1.3 in cat spinal cord: light and electron microscopic immunohistochemical study. J Comp Neurol. 2008, 507: 1109-1127. 10.1002/cne.21595. CAS PubMed Google Scholar Lee RH, Heckman CJ: ...
by excited of different light from the laser scanning confocal microscope, fluorescence of MSCs nuclear labelled DAPI showed blue fluorescence, some MSCs of blue nuclear expressed Flk-1 protein and showed red fluorescence, then part of the Flk-1 positive MSCs expressed vWF protein and showed green...
light). (d) STXM-based compositional image of mineralizedPhormidiumsp. NEXAFS analysis spot is indicated (spot 1). (e) Bright-field TEM image showing a mineralized empty sheath ofPhormidium. (f) Magnified region from (e). NEXAFS analysis spots (spots 2 and 3) and selected area electron ...
Mice were kept in a pathogen-free environment with a controlled light cycle from 6:00 h to 18:00 h, a temperature of 20 to 23 °C and humidity of 50 to 60%. Mice were allowed to acclimatize for a minimum of 7 days before each experiment....