Simple and Differential Staining of BacteriaUnknownMississippi University for WomenMy Publications
THE common methods for differential staining of lignified plant tissue are specific when used correctly, but minor errors in procedure may lead to unsatisfactory results. The following simple technique has been found a reliable, cheap and quick supplement to the other methods available, and to give...
The essential steps of the 33258 Hoechst-Giemsa method for differential chromatid staining consist of (1) 33258 Hoechst treatment, (2) exposure to light, and (3) Giemsa staining. The staining was shown to be a function of the concentration of 33258 Hoechst and the light exposure. The dye wa...
Differential Staining of Kinetochores in the Somatic Metaphase Chromosomes of Allium Cepa by a Simple Aceto-Orcein TechniqueAloe vera, Tradescantia virginianaWithout any elaborate post-fixation treatment, dot-like kinetochores in the somatic metaphase chromosomes of Allium cepa were stained differentially ...
I H&E staining in rectum GIL. J-L CD117 (J) and DOG1 (K) were negative, but diffuse strong positive SMA (L) in rectum GIL. (Magnification: 200 ×). Full size image Special stains diagnosis of GISTs versus GILs The GISTs approximately account for 92.3% were stained blue with MT ...
GFAP staining showed a widespread increase in the astrocyte activity in septic mice (regardless of outcome) compared to healthy controls; it was most pronounced in the cerebellum. The outcome-based comparison demonstrated an exacerbated astrocyte activation only in the medulla oblongata of P-DIE mice...
Following primary antibody staining, embryos were further washed (4 × 10 min) in PBTx, blocked in PBT-B at room temperature for 1 h, and incubated with goat anti-rabbit IgG secondary antibody conjugated with Alexa Fluor™ 488 (Invitrogen, A11034, 1:500 (v/v) dilution in PBT...
Conclusion: This study confirms 100% expression of DOG 1 in cases of chondroblastomas but intensity and proportion of staining pattern was variable. Therefore DOG 1 may prove to be a useful immunohistochemical marker for diagnosis of chondroblastomas in the future in difficult cases in correlation...
The scores for each protein were dichotomised using the following cutoff points; negative staining vs positive staining, negative and weak staining vs moderate and strong staining, and strong staining vs negative/weak/moderate staining. Further details of data analysis and statistics are provided in...
Variation in the size and number of axial segments underlies much of the diversity in animal body plans. Here we investigate the evolutionary, genetic and developmental mechanisms driving tail-length differences between forest and prairie ecotypes of dee