Blood samples collected into separate sterile vials without Ethylenediaminetetraacetic acid (EDTA) for serum collection. Serum samples were collected in sterile vials. Few drops of 1:10,000 sodium azide solution was added to the serum samples and stored at –20 °C until use. Wet blood film ...
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In this study, this was the case for FFPE samples, but OCT samples needed to be transferred to new vials, which could introduce potential losses of material. Table 1 Number of quantifiable proteins in tumour samples from different patients preserved using different methods. Full size table...
Clinical specimens were inoculated in Bactec Plus anaerobic/aerobic vials and analysed in a Bactec 9240 continuous-monitoring system (Becton-Dickinson Microbiology System, Cockeysville, MD, USA). Corynebacterium-like colonies were selected for identification when they were grown in significant numbers (>...
The supernatant was transferred to a new tube and frozen at −20 °C for at least 2 h. Finally, the top layer (organic phase) was filtered through 0.2 μm regenerated cellulose filters into 1.5 mL analysis vials. Quantitative analysis for parent BEA or ENNB were performed with an ...
Further research should be carried out to confirm the potential negative effect of the preservatives used in multidose heparin vials.doi:10.1016/j.jevs.2019.01.015Cuervo-Arango, JuanClaes, Anthony N.Beitsma, MabelStout, Tom A.E.Elsevier Science...
Clinical specimens were inoculated in Bactec Plus anaerobic/aerobic vials and analysed in a Bactec 9240 continuous-monitoring system (Becton-Dickinson Microbiology System, Cockeysville, MD, USA). Corynebacterium-like colonies were selected for identification when they were grown in significant numbers (>...
The reaction system (20 μL) consisted of the following: 1 μL of template cDNA, 10 μL each of the upstream and downstream primers, and 5 ml (5 × 1 mL vials) of RNase-free water. The thermal cycling procedure was as follows: 94 °C for 10 min, 94 °C for 30 s, 60 ...
Ovarian tissue was frozen according to the method described for bovine ovaries [16]. Briefly, each ovarian sample was placed into a 1.2 ml cryogenic vial containing 1.0 ml of cryoprotectant solution, and frozen using a programmable freezer (Dominium K, Biocom, Brazil). Vials were equilibrated at...
Next, cardia and noncardia biopsy specimens were dissected by an experienced endoscopy physician with sterile forceps under strict procedures and then placed into sterile tubes (2.0-mL cryogenic vials; Corning Incorporated, New York, NY, USA). Esophageal swab specimens were collected using sterile ...