The tissue samples were rinsed in phosphate-buffered saline containing 1% penicillin-streptomycin until the rinse solution was clear. The collected mammary gland parenchima tissue was vitrified and stored at -80 °C for subsequent experimental use. MECs culture The mammary gland tissue samples frozen ...
influenced by the absence of sulfolipids and sulfatides from the cell wall. Mannosylated lipoarabinomannan (ManLAM), a prominent glycolipid inM. bovisis a ligand for interactions with dendritic and macrophage cells, allowing it to enter phagocytes via mannose receptors. LAM is a potent scavenger fo...
types of antibiotics—including ampicillin/penicillin and mater- nal vaginal metronidazole—and CHD (21–23). Mechanisms for the effect of other antibiotics on birth defect are not clear. Besides direct effects of antibiotics on CHD, the underlying infection of urogenital tract or other area should...
Each sample (0.25 g) was extracted for metagenomic (microbial genomic) DNA using a DNeasy PowerSoil Kit, following the manufacturer's instructions (Qiagen, Maryland, USA). The quantity and quality of the extracted metagenomic DNA was analysed by 1% (w/v) agarose gel electrophoresis and nanodrop...
Murine melanoma cells (B16F10) (Cell Resource Center of the Shanghai Institute for Biological Sciences, Shanghai, China) were propagated in Dulbecco's modified Eagle's medium (DMEM, Gibco) augmented with 10% foetal bovine serum (FBS, Gibco, MA, USA), 100 U/mL penicillin, and 100 μg/mL...
BT-549 and MCF-7 cells were cultured in full medium containing RPMI 1640 (Gibco), 10% heat-inactivated fetal bovine serum (FBS), 100 units/ml penicillin, and 100 μg/ml streptomycin. BT-549 and MCF-7 cells were obtained from the American Tissue Culture Collection (ATCC). KA-resistant ...
In today's world, we have a huge amount of debt outstanding. Academic researchers Carmen Reinhart and Kenneth Rogoff have become famous for their book This Time is Different: Eight Centuries of Financial Folly and their earlier paper This Time is Differe
Sequence analysis showed that the epitope sequences recognized by 1D6, 1H2, and 3F2 are highly conserved (98%) among RHDV1 strains, whereas the epitope sequences recognized by 1G2, 2C1, 3B7, and 5D6 are 100% conserved among RHDV2 strains. The high conservation of the epitope ...
Human BM-MSCs from two independent donors (one male and one female, PromoCell, Heidelberg, Germany) were amplified in PromoCell Growth Medium supplemented with 1% penicillin–streptomycin (P/S) (Invitrogen, Paisley, UK) in a humidified atmosphere at 37 °C, 5% CO2(Air Liquide, Paris, France...
(FNC)-coated plastic culture dishes and grown until they reached confluence in a proliferation medium (Opti-MEM-I medium supplemented with 8% fetal bovine serum, 5 ng/mL epidermal growth factor, 0.08% chondroitin sulfate, 20 μg/mL ascorbic acid and 100 IU/mL of penicillin and 100 μg/mL...