Filter samples were taken to assess the oxidant generation associated with PM by an optimized dithiothreitol (DTT) method. Diesel exhaust PM had a higher ROS response when it was in the presence of an urban hydrocarbon mixture and was associated with significant O 3 production. For all the ...
In order to reduce the image analysis errors, a minimum life-time threshold of two successive frames after creation (before disappearance) is imposed on a signal to consider the event as a rescue i(inncaFtteaigrs.tm r1osCpo.hBfeo)dtt,hrwaNnhsreiatrineodnthN. Techiinneccnrrueemaassbee...
R e s u lts Inhibition of in vitro TGase activity by cystamine or cysteamine To compare the difference of inhibition characteristics between cystamine and cysteamine in a reducing condition, we first examined the effects of reducing agents on in vitro TGase activity. Dithiothreitol (DTT) or ...
Cells were lysed in lysis buffer containing 1% Triton X-100, 150 mM NaCl, 2 mM MgCl2, 2 mM CaCl2, and complete mini protease inhibitor cocktail (Roche), and 20 mM DTT was added (samples for immunoblotting with mouse anti-podocalyxin (R&D Systems MAB1658) were not treated with DTT). Nu...
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After centrifugation of the homogenates and the hemolymph (16,000×g for 5 min at 4 °C), the supernatants were placed in a new tube. 160 µL of the supernatants were diluted with 5x sample buffer (0.1 M TRIS (pH = 6.8), 0.5 M DTT, 2% SDS, 10% glycerol, 1 mM EDTA, ...
different types of viral pathogen can disrupt synergistic antiviral immunity mediated by SA and JA via OsNPR1 in the monocot rice. The P2 protein of rice stripe virus, a negative-stranded RNA virus in the genusTenuivirus, promotes OsNPR1 degradation by enhancing the association of OsNPR1 and ...
Additional File 1: Table S1: PM characterization of coarse, fine and ultrafine PM sampled in two different road tunnels (Marienborg, and Hell) under humid road surface conditions; a) Total- and organic carbon (OC), endotoxin content and oxidative potentials (OPDTT) b) Hydrodynamic size distrib...
. After post-translational cleavage, α2δ subunits are re-attached by disulfide bonds between the α2and the δ subunit. Therefore, we predicted only the larger band in the absence of DTT because the δ moiety would not be removed. This was indeed the case (Fig.1A, left blot, right ...
27. Cell pellets were lysed with RIPA buffer (50 mM Tris, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 0.1% sodium azide, 1 mM PMSF, 2 mM sodium orthovanadate, 2 mM DTT and freshly added 1 × protease inhibitor, pH 8.0). Samples were snap-frozen...