TGase 2 (10 ng of purified protein) was add- ed to each well which contains 5-(biotinamido)pentyla- mine (BP; 0-20 µM) in the 100 µl of a buffer (50 mM Tris-Cl, pH 7.5, 100 mM NaCl, 10 mM CaCl2 and 0.1% Triton X-100). In experiments under reducing conditions, ...
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Staining was achieved semiautomatically (Techmate 500, DAKO) using a streptavidin-biotin variant and diaminobenzidine was used as chromagen. Sodium Bisulfite DNA Treatment DNA was isolated from frozen samples using the DNAzol reagent kit as specified by the manufacturers (Molecular Research Center Inc...
According to the result thatT. bernardiireached the highest biomass at a glucose concentration of 60 g/L, we investigated the effects of different NaNO3concentrations on the biomass, lipid content, and lipid productivity ofT. bernardiiat the glucose concentration of 60 g/L (Fig.2). When the ...
Mean daily biotin flow was 0.48±0.11mg/kg FOM without any systematic effect of diet composition. The ruminal biotin balance, calculated as the difference between biotin flow at the duodenum and biotin intake, was positive (1.4–2.0mg/d) in cows fed the mixed roughage/concentrate diets and ...
(clone eBio1D3), CD137-Biotin (clone 17B5), DR5-Biotin (clone MD5.1), Streptavidin-Pe-Cy7 (all from eBioscience, Kensington, Australia), Ly-6G-APC-Cy7 (clone 1A8), H-2K[d]-Biotin (clone SF11-1.1), CD40-Biotin (clone 3/23) (all from BD Biosciences, Sydney, Australia) and ...
protein TDP43 (pTDP43), α-synuclein, and/or ubiquitin as listed in Additional file1: Table S1. Primary antibodies were detected with biotinylated secondary antibodies and visualized with the DABMap Kit (Ventana, USA) or with diaminobencidine-HCl and the avidin-biotin complex (Vector, USA)....
The vitamin premix provided (per kilogram of diet): 12,000 IU of vitamin A, 2,400 IU of vitamin D3, 40 mg of vitamin E, 31.8 mg of vitamin K, 2.5 mg of vitamin B1, 4.0 mg of vitamin B2, 2.0 mg of vitamin B6, 15 µg of vitamin B12, 60 µg of biotin, 30 mg of niac...
A dilution series of CEA samples was incubated with aliquots of the particle mixture and secondary biotin-streptavidin-phycoerythrin-conjugated antibody directed against a different epitope on the CEA molecule. The fluorescence intensity of the two particle types was measured flow cytometrically, and a ...
A dilution series of CEA samples was incubated with aliquots of the particle mixture and secondary biotin-streptavidin-phycoerythrin-conjugated antibody directed against a different epitope on the CEA molecule. The fluorescence intensity of the two particle types was measured flow cytometrically, and a ...