Examples of these kinds of bullets include: airgun pellets, blackpowder rifle bullets, Minie balls, Foster-type shotgun slugs (HB, SWC nose), pistol wadcutters and semi-wadcutters.A lead bullet formed in a single die will have a shoulder between shank and nose, created because this kind ...
The pellets were resuspended and incubated with 0.2 mg/ml Thioflavin S for 2 h at room temperature under stirring. Then, fibrils were washed by centrifugation and resuspended in freshly prepared buffer three times to remove excess of Thioflavin S. The fibrils obtained were finally resuspended...
RNA was extracted from the cell pellets with the Artrum Total RNA Mini Kit (Bio-Rad, Veenendaal, The Netherlands cat No. 732-6820) according to the manufacturer's recommendations. The extracted total RNA was treated with the TURBO DNA-free Kit to remove DNA (Ambion, Nieuwerkerk a/d lJs...
Bayramoglu G, Arıca MY (2008) Removal of heavy mercury (II), cadmium (II) and zinc (II) metal ions by live and heat inactivated Lentinus edodes pellets. Chem Eng J 143:133–140 CAS Google Scholar Beveridge T, Hughes M, Lee H (1997) Metal-microbe interactions: contemporary approache...
(apply short spin for residual liquid) and pellets were carefully air-dried for 5 minutes (this step can be repeated to pool material from 2 or more gradients). In order to isolate total RNA, polysome isolate corresponding to 150 μg RNA was directly mixed with guanidine hydrochloride and ...
Focused electron beam induced deposition (FEBID) is a versatile tool to produce nanostructures through electron-induced decomposition of metal-containing precursor molecules. However, the metal content of the resulting materials is often low. Using diffe
Beans are widely consumed throughout the world, rich in non-nutrient phenolic compounds and other bioactive constituents, including alkaloids, lectins, and others. However, research about in vitro digestion impacts on the changes of bioactive compounds’
NaOH pellets (Sigma Aldrich, Milan, Italy (ACS reagent)) were added to demineralized water to obtain different concentrations: E1 solution has a concentration of 10−2 mol NaOH/L with a pH = 12, while E2 has a concentration of 10−5 mol NaOH/L and a pH equal to 9. In order to...
Finally, the cleaned pellets were dried under air flow in a fume hood for 1 h, reconstituted in 100 µL of shotgun buffer (8 mol/L urea, 2 mol/L thiourea, 0.15% AALS II in 100 mmol/L tris-HCl, pH 7.5), and protein contents were determined by 2-D Quant Kit (GE Healthcare,...
The spectra of the solids were taken with KBr pellets. 2.2.4. Surface Tension Measurement The surface tension of the NaOL solution with different concentrations was performed by the Wilhelmy plate method, using the K100MK2 tensiometer (Kru˝ ss GmbH, Hamburg, Germany). The desired amount of ...