After drying at 150 °C for 2 h, the NiO-GDC substrate powder was co-pressed with GDC power under ~180 MPa into button-like pellets, followed by the calcination at 1450 °C for 7 h. The thickness of the GDC electrolyte layer was 13−18 μm. The cathode slurry was s...
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The pellets were resuspended and incubated with 0.2 mg/ml Thioflavin S for 2 h at room temperature under stirring. Then, fibrils were washed by centrifugation and resuspended in freshly prepared buffer three times to remove excess of Thioflavin S. The fibrils obtained were finally resuspended...
Tubes were centrifuged at 3400 × g and 4°C for one hour, supernatant was removed entirely (apply short spin for residual liquid) and pellets were carefully air-dried for 5 minutes (this step can be repeated to pool material from 2 or more gradients). In order to isolate total RNA...
DNA extraction was performed from the pellets using the DNeasy PowerSoil Pro Kit (Qiagen, Hilden, Germany), according to the manufacturer's instructions, adopting a modified version of the standard protocol previously optimized and validated to increase the total microbial DNA yield from food ...
RNA was extracted from the cell pellets with the Artrum Total RNA Mini Kit (Bio-Rad, Veenendaal, The Netherlands cat No. 732-6820) according to the manufacturer's recommendations. The extracted total RNA was treated with the TURBO DNA-free Kit to remove DNA (Ambion, Nieuwerkerk a/d lJs...
Finally, the cleaned pellets were dried under air flow in a fume hood for 1 h, reconstituted in 100 µL of shotgun buffer (8 mol/L urea, 2 mol/L thiourea, 0.15% AALS II in 100 mmol/L tris-HCl, pH 7.5), and protein contents were determined by 2-D Quant Kit (GE Healthcare,...
Cai et al. fabricated polyaniline/graphene nanosheets (PANI/GNs) TE composite as pellets and films. Electrical conductivity and the Seebeck coefficient rose synchronously with an increased amount of graphene. Increasing carrier mobility seems to be a method with immense potential to make efficient ...
Fluids 2019, 4, 7 3 of 12 Before the melt-mixing process, polymer powder was prepared from pellets using a Mill Retsch® ZM 200 (Haan, Germany) and both polymer powder and MWCNT were properly dried. PP was dried at T = 80 ◦C in a vacuum oven during 2 h and MWCNT were dried...
Approximately one gram of oven-dried ground sample with a particle size ranging from 20 to 40 mesh was converted into pellets using a hydraulic pellet press and loaded into a model 6300 oxygen bomb calorimeter (Parr, Moline, IL, USA). Before the analysis of the samples, the calorimeter was...