Moreover with the great sensitivity and dynamic range that is afforded by these dyes, 2D DIGE can give greater accuracy of quantitation than silver staining (2). It has been reported that the correlation between quantitation by 2D DIGE and metabolic stable isotope labeling is exceptionally good (...
between the master and PDMS replicas43. The mixture of the PDMS prepolymer (sylgard 184, part A) and the curing agent (sylgard 184, part B) with a 10:1 weight ratio was evenly stirred for 10 min using a glass rod. The PDMS mixture was then poured onto a SU-8/silicon master. The ...