Before looking at the difference between endonuclease and exonuclease, it is important to know what exactly a nuclease is. A nuclease is an enzyme capable of cleaving phosphodiester bonds betweennucleotidesinnu
Exonucleases are enzymes that work by cleaving nucleotides one at a time from the end (exo) of a polynucleotide chain. A hydrolyzing reaction that breaks phosphodiester bonds at either the 3′ or the 5′ end occurs. Endonuclease Any of a group of enzymes that catalyze the hydrolysis of bonds...
while exonuclease is a nuclease enzyme that cleaves the nucleotides in a polynucleotide from either 5’ end or 3’ end one at a time. So, this is the key difference between restriction endonuclease and exonuclease. Moreover, restriction endonuclease...
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