They suggested that the differences in agonist-induced internalization between β1- and β2ARs are partially because of the proline-rich region in the third intracellular loop of β1AR. Thus, no study has been conducted to analyze the interaction of β1AR with β-arrestin explaining the ...
Furthermore, in a recent study evaluating the genotype and phenotype for 1017 healthy blood donors not treated with thiopurine drugs, only the TPMT genotype and SAM levels in red blood cells (RBC) were found to influence TPMT activity25. The correlation between TPMT genotype and SAM levels is ...
coli BL21(DE3)RIPL (Novagen, Madison, WI, USA). Freshly transformed cells were grown in high salt LB medium with 100 µg/mL ampicillin and 34 µg/mL chloramphenicol (Panreac-AppliChem, Darmstadt, Germany) at 37 °C, until the OD600 value reached 0.8. Then, expression was induced ...