et al. Reprogramming of intestinal glucose metabolism and glycemic control in rats after gastric bypass. Science 341, 406–410 (2013). Article ADS CAS PubMed PubMed Central Google Scholar Troy, S. et al. Intestinal gluconeogenesis is a key factor for early metabolic changes after gastric ...
Total RNA was extracted from LT muscle (100 mg) using a Trizol reagent (Vazyme, Nanjing, China) according to the manufacturer's instructions. The RNA integrity was assessed using 1% agarose gel electrophoresis. The quality and quantity of RNA samples were determined using a spectrophotometer (DeNo...
Briefly, small RNAs were extracted from the mouse liver using miRNeasy Mini kit (QIAGEN, Cat# 217004) according to the manufacturer’s instructions. A 100-μL mixture consisting of 20 μg of small RNA and 10 mM NaIO4was incubated at 0 °C for 40 min in the dark. The oxidized...
After the low pH treatment, the cells were harvested by mild trypsin digestion. The FITC-Annexin V and propidium iodide were used for double staining in accordance with the manufacturer’s instructions, followed by Gallios flow cytometry (Beckman Coulter). Kaluza for Gallios acquisition software (...
Diets should only be started on an outpatient basis if there has been a thorough teaching program prior to commencement, including instructions on the daily home monitoring of blood or urine ketones and the management of possible early side effects, such as excess ketosis and hypoglycemia. KD ...
After the treatment, cell viability was analyzed by the Cell Counting Kit-8 (Dojindo, CK04) according to the manufacturer's instructions. The absorbance at 450 nm was read on a SpectraMax M2e (Molecular Devices). Lactate dehydrogenase (LDH) cytotoxicity assay The PGECs were cultured and then ...
Diets should only be started on an outpatient basis if there has been a thorough teaching program prior to commencement, including instructions on the daily home monitoring of blood or urine ketones and the management of possible early side effects, such as excess ketosis and hypoglycemia. KD ...
Changes in dietary intake, food tolerance, hedonic hunger, binge eating problems, and gastrointestinal symptoms after sleeve gastrectomy compared with after gastric bypass; 1-year results from the Oseberg study-a randomized controlled trial. Am. J. Clin. Nutr. 2023, 117, 586–598. [Google ...
The colonic microbial DNA was extracted by the instructions of MOBIO power fecal DNA extraction kit and colonic microbial 16S rDNA fragments were analyzed by Chengdu Luoning Biological Technology Co. (Chengdu, China). The V4 region of colonic microbial 16S rDNA was amplified by specific primers ...
DNA methylation screening after roux-en Y gastric bypass reveals the epigenetic signature stems from genes related to the surgery per se. BMC Med. Genom. 2019, 12, 72. [Google Scholar] [CrossRef] [PubMed] Nitert, M.D.; Dayeh, T.; Volkov, P.; Elgzyri, T.; Hall, E.; Nilsson, ...