Human embryonic kidney 293 (HEK293) cells were maintained in suspension culture with Freestyle medium (Gibco 12338018) supplemented by 1% heat-inactivated fetal bovine serum (Sigma F2442) at 37 °C, 5% carbon dioxide and 70% humidity as previously described46. For all transient transfections,...
In this study, firstly, the mathematical models of displacement and torque factor of the horsehead suspension point of the pumping unit have been established based on the easily measured beam inclination angle. Secondly, by analyzing the motion law and the energy flow mechanism of the beam pumping...