Per well, 20 μl of the antibody cocktail was added and incubated for 1 h at room temperature. For imaging, a PerkinElmer Opera Phenix automated spinning-disk confocal microscope was used and each well of a 348-well plate was imaged at 20× magnification with 5 × 5 non-...
about the functional roles of cell types in motion, object and colour vision. Connectivity with ‘boundary types’ that straddle the optic lobe and central brain is also quantified. We showcase the advantages of connectomic cell typing: complete and unbiased sampling, a rich array of features bas...