Forest City Diagnostic Imaging Does Whatever It Takes To Deliver The Highest Quality MRI, CT Scans, X-Ray, & Ultrasound Services In The Rockford, IL Area.
Forest City Diagnostic Imaging Does Whatever It Takes To Deliver The Highest Quality MRI, CT Scans, X-Ray, & Ultrasound Services In The Rockford, IL Area.
Protein bands were visualized using secondary antibodies conjugated to HRP (1:3000, BioRad) followed by incubation with Pierce SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Scientific, Rockford, Ill.) and analyzed using the G:BOX gel documentation system (Syngene). Tetradecanoylphorbol ...
All patients were admitted to the study to localize or rule out the presence of inflammation/infection. Imaging studies and follow up care took place at Harbor-UCLA Medical Center, Torrance, CA. In all cases, the site(s) of inflammation/infection were identifiable by gamma scintigraphy within t...
Membranes were washed three times for 15 min at room temperature using TBST and subjected to ECL Western blotting substrate (32106, Pierce, Thermo Scientific, Rockford, IL, USA) for 30 s in the dark before exposure (1708370, ChemiDoc Touch Imaging System, Biorad Laboratories, Hercules, CA, ...
After washing, the reactivity on the membranes was detected with an ECL Western blot detection kit (Pierce, Rockford, IL). To align Coomassie-stained gels with immunoblot images, gel images were acquired with a GS-800 calibrated imaging densitometer (Bio-Rad, Hercules, CA). The spot detection...
The capture antibodies to be printed onto microarray slides were purified by dialysis (Slide-A-Lyzer; Pierce Biotechnology, Rockford, IL) to 1× PBS and ultracentrifuged. Biotinylation was performed using the EZ-Link-sulfo-NHS-LC-Biotin kit (Pierce Biotechnology) according to the manufacturer’s ...
The protein concentration was determined using a bicinchoninic acid (BCA) Protein Assay Reagent (Thermo Fisher Scientific, Rockford, IL, USA). Denaturation, reduction, trypsin digestion, iTRAQ labeling, and strong cation exchange (SCX) chromatography Sample preparation was performed accordingly to the ...
The rapid progress of proteomics over the past years has allowed the discovery of a large number of potential biomarker candidates to improve early tumor diagnosis and therapeutic response, thus being further integrated into clinical environment. High gr
Cell-free protein synthesis (CFPS) represents a versatile key technology for the production of toxic proteins. As a cell lysate, rather than viable cells, is used, the toxic effects on the host organism can be circumvented. The open nature of cell-free s