Diagnostic parameters for visceral leishmaniasis (VL), a potentially fatal parasitic disease caused by Leishmania donovani, have been redefined in the last decade with the development of serological and molecular tests, though a definitive diagnosis still banks on the century-old parasitological methods...
Visceral leishmaniasis (VL), a deadly parasitic disease, is a major public health concern globally. Countries affected by VL have signed the London Declaration on Neglected Tropical Diseases and committed to eliminate VL as a public health problem by 2020. To achieve and sustain VL elimination, i...
Visceralleishmaniasis: Dot-enzyme immunoassay; Circulating antigen 1.Introduction Visceralleishmaniasis (VL)isadiseasecausedby Abbreviations: VL,visceralleishmaniasis:EIA,enzyme im- munoassay; MAb.monoclonal antibody; CL.cutaneous leishma- niasis:OD,opticaldensity;PBS,phosphate buffered saline. ICorresponding...
Visceral leishmaniasis (VL) is a major public health problem in the eastern states in India, namely, Bihar, West Bengal, Jharkhand, and Eastern Uttar Pradesh. Bihar alone accounts for >90% of the total number of cases of kala-azar reported in India. The disease is predominantly found in ...
Sensitive, reliable and fast diagnostic tools that are applicable in low-resource settings, at the point of care (PoC), are seen as crucial in the fight against visceral leishmaniasis (VL) and cutaneous leishmaniasis (CL). Addressing the need for a PoC test, several diagnostic tests, includin...
The performance of the beta-ME ELISA thus developed was assessed in patients with confirmed visceral leishmaniasis (VL), revealing slightly lower sensitivity (39/40=97.5%) than that of the DAT (40/40=100%). When challenged with sera of individuals with non-VL conditions, including leukaemia ...
REVIEW Visceral leishmaniasis: what are the needs for diagnosis, treatment and control? François Chappuis*‡, Shyam Sundar§, Asrat Hailu||, Hashim Ghalib¶, Suman Rijal#, Rosanna W. Peeling¶, Jorge Alvar** and Marleen Boelaert‡‡ Abstract | Visceral leishmaniasis (VL) is a ...
The micro-ELISA technique in the serodiagnosis of visceral leishmaniasis The micro-ELISA test using alkaline phosphatase conjugate was applied to sera from cases of human and canine visceral leishmaniasis originating in the sout... M.,Hommel,W.,... - 《Annals of Tropical Medicine & Parasitology...
BACKGROUND: The diagnosis of visceral leishmaniasis (VL) in patients with fever and a large spleen relies on showing Leishmania parasites in tissue samples and on serological tests. Parasitological techniques are invasive, require sophisticated laboratories, consume time, or lack accuracy. Recently, ra...
Background: Diagnosis of visceral leishmaniasis (VL) through the detection of its causative agents namely Leishmania donovani and L. infantum is traditionally based on Giemsa-stained smears of bone marrow, spleen aspirates, liver or lymph node. Collection of these samples involve invasive procedures...