Protein #4 (DIABLO) was extracted from the gel for peptide analysis. (B) Capillary column RP-HPLC/ESI-MS/MS of a tryptic digest of DIABLO. The unfractionated peptide mixture (30 μL, ∼0.2 pmol from a total volume of ∼100 μl) was applied to the 0.2 mm ID column at 6 μl/...