The Paleolithic Diet: “More randomized trials need to be done to highlight the consequences of such diets that eliminate one or more food groups. PD is powerful at advancing weight reduction for the time being but its efficacy in cardiovascular events is not well established as limited long-te...
After 48 h, Th22 cells were added to BV2 cells in the presence of 50 mmol/L glucose, after which the plates were cultured for 48 h at 37 °C in a 5% CO2 atmosphere. The cells were then divided into 6 groups: the control group (complete DMEM), mannitol group (complete DMEM ...
Intracellular ROS levels were evaluated using the DCFH-DA probe (S0033, Beyotime, Shanghai, China), and the fluorescence intensity indirectly reflects the degree of oxidative stress in cells. HK-2 cells were seeded in 6-well plates and given different treatments for 48 h. After adding the DCF...
By accepting optional cookies, you consent to the processing of your personal data - including transfers to third parties. Some third parties are outside of the European Economic Area, with varying standards of data protection. See our privacy policy for more information on the use of your perso...
Neutrophils (1 × 105 cells) were plated in 24-well plates with either 5.5 mmol/L (control) or 22 mmol/L glucose (high glucose) to establish the hyperglycaemic cell model. Induction and extraction of neutrophil extracellular traps Neutrophils (1 × 105 cells) were cultured in 24-...
The introduction of vegf-a through lentivirus resulted in notable enhancements in the proliferation, motility, viability, and permeability of ARPE-19 cells. For cell proliferation assessment, ARPE-19 cells were initially seeded in 12-well plates at a density of 3,000 cells per well, followed by...
Serum concentrations of insulin were measured using rat insulin ELISA kits. General procedures followed the manufacturer's instructions. Plates were read at OD450 on an enzyme-linked immunosorbent assay plate reader (BIO-RAD, USA). Data analysis A population approach was applied to investigate the ...
HK-2 cells were cultured in 24-well plates, fixed with 4% paraformaldehyde for 15 min after administration, and infiltrated with 0.5% Triton X-100 for 10 min. After blocking with 10% goat serum for 1 h, the cells were incubated overnight at 4 °C with primary antibodies of α-SMA (...
Diabetic cardiomyopathy (DCM) is a serious health-threatening complication of diabetes mellitus characterized by myocardial fibrosis and abnormal cardiac function. Human umbilical cord mesenchymal stromal cells (hUC-MSCs) are a potential therapeutic tool
but they have to be monitored and accounted for. Overdoing it can really cause problems, so remember to include carbohydrates in moderation. It is a good idea to focus on filling your plates with lots of healthy, fresh produce and lean protein and choosing a healthy carbohydrate to accompany ...