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Journal of Biomedical Science (2022) 29:17 Page 4 of 17 non-fat skim milk (Blocking-Grade Blocker; Bio-Rad) in 1× TBST (10 mM Tris–HCl, 150 mM NaCl, 0.05% Tween-20) at room temperature for 30 min and incu- bated with primary antibodies at 4 °C overnight. The...