蛋白别名: 4-hydroxy-2-oxoglutarate aldolase, mitochondrial; DHDPS-like protein; Dihydrodipicolinate synthase-like; dihydrodipicolinate synthase-like, mitochondrial; dihydrodipicolinate synthetase homolog 2; N-acetylneuraminate pyruvate lyase 2 (putative); Probable 2-keto-4-hydroxyglutarate aldolase; probab...
Lysine biosynthesis in bacteria and plants commences with a condensation reaction catalysed by dihydrodipicolinate synthase (DHDPS) followed by a reduction reaction catalysed by dihydrodipicolinate reductase (DHDPR). Interestingly, both DHDPS and DHDPR exist as different oligomeric forms in bacteria and...
目的克隆红花赖氨酸生物合成途径关键酶二氢吡啶二羧酸合酶(dihydrodipicolinate synthase DHDPS)基因的全长cDNA序列,构建植物超表达载体。方法根据红花转录组文库注释信息获得红花DHDPS(CtDHDPS)基因的中间序列,通过RT-PCR与RACE技术从红花种子中克隆CtDHDPS基因全长cDNA序列。采用DNA重组技术构建pBASTA-CtDHDPS植物超表...
筛选标记生物安全从烟草(Nicotiana tabacum L.cv)中克隆得到赖氨酸合成过程中的关键酶基因二氢吡啶二羧酸合酶(dihydrodipicolinate synthase,DHDPS)基因(dhdps),对其进行体外分子改造,以解除末端产物对它的反馈抑制。将突变基因用做转基因植物的筛选标记转化烟草,用赖氨酸类似物(S-2-氨基乙基-L-半胱氨酸,AEC)作为...
Soares da Costa1, Pierre Faou1, F. Grant Pearce2, Santosh Panjikar3,4 & Matthew A. Perugini1 Lysine biosynthesis in bacteria and plants commences with a condensation reaction catalysed by dihydrodipicolinate synthase (DHDPS) followed by a reduction reaction catalysed by ...
dihydrodipic01inatesynthase,were clonedfromArabidopsis thaliana andNicotianatabacum三.cv respectivelythen amolecularreformweremadeinvitrotoreliefthe feedback suppression orinhibition,andaresearchaboutthe possibility of using themutategenes asselectablemarkerswas made,analog ofmethionineor lysineasselectable reagent....
Dihydrodipicolinate synthase (DHDPS) is a validated antibiotic target for which a new approach to inhibitor design has been proposed: disrupting native tetramer formation by targeting the dimer鈥揹imer interface. In this study, rational design afforded a variant of Mycobacterium tuberculosis, Mtb-DHD...
Ligand-Induced Changes in Structure and Dynamics of the Dihydrodipicolinate (DHDPS) Synthase Enzyme Complex Studied by HDX-MSProtein-ligand interactions usually induce a reduction in the measured HDX rates, consistent with the behavior seen here for DHDPS. HDX shows that the protection patterns of...
Lysine is one of the most limiting amino acids in plants and its biosynthesis is carefully regulated through inhibition of the first committed step in the pathway catalyzed by dihydrodipicolinate synthase (DHDPS). This is mediated via a feedback mechanism involving the binding of lysine to the ...
Cloning and characterization of the DHDPS gene encoding the lysine biosynthetic enzyme di- hydrodipoc olinate synthase from Zizania latifolia (Griiseb). Plant. Mol. Biol. Rep. 27: 199-208.KONG, F.; JIANG, S.; MENG, X.; SONG, C.; SHI, J.; JIN, D.; JIANG, S.; WANG, B. ...