Peripheral blood analysis of CD45.1/CD45.2 expression showed that recipient mice were generally 96% reconstituted with donor cells. Antibiotic-laced drinking water (0.2% w/v neomycin) was administered ad libitum for 72 h before irradiation and for 2 weeks after BM reconstitution. Cm stimulation ...
In the early morning period, the donor manually pumped 3–5 ml of breast milk into a sterile, RNase-free collection tube, which was immediately placed on ice. Subsequent procedures of MFG isolation were finished within 3 h, with samples then transferred to a −80 °C freezer. We employed...