Equivocal sequences that are predicted to be 伪-helical from amino acid preference but are found to be -strand from x-ray diffraction of their respective proteins, and equivocal sequences that are predicted to be -strand but found to be a-helical, are shown by circular dichroism (CD) to ...
Active nuclear import of Ran exchange factor RCC1 is mediated by importin α3. This pathway is essential to generate a gradient of RanGTP on chromatin that directs nucleocytoplasmic transport, mitotic spindle assembly and nuclear envelope formation. Here
Special AT-Rich Sequence-Binding Protein 2 (Satb2) is expressed in neurons of all layers and is indispensable for the formation of the corpus callosum12,13. In the murine brain, Satb2-expressing cells are born during a protracted period of development14, including both earlier- and later-...
Replacement of either TRAP220 LXXLL motif with the corresponding 13 amino acids of SRC1 LXM2 strongly enhanced the interaction of the TRAP220 NID with the estrogen receptor. Mutational analysis revealed combinatorial effects of the LXM1 core and flanking sequences in the determination of the NR ...
The complete amino acid sequence of a fatty acid-binding protein from human heart was determined by automated Edman degradation of CNBr, BNPS-skatole [3'-b... GD Offner,P Brecher,WB Sawlivich,... - 《Biochemical Journal》 被引量: 148发表: 1988年 Cross-beta Order and Diversity in Nanoc...
Responses to these hormones are mediated by transcription factors of the bri1-EMS suppressor 1/brassinazole resistant 1 subfamily, and BRs activate these factors by impairing their inhibitory phosphorylation by GSK3/shaggy-like kinases. Here we show that BRs induce nuclear compartmentalization of ...
For the C-type adenovirus (serotypes 2 and 5), these mRNAs encode proteins of 243 and 289 amino acids, respectively (Fig. 1 A). Amino acid sequences of E1A isoforms from each of the adenovirus serotypes differ substantially, except within CRs1 that act as interaction motifs for E1A ...
The isolated cDNA, termed LST-2, encodes 702 amino acids (Mr 77,398). The hydrophobicity analysis20 suggests the presence of 12 transmembrane domains, which is characteristic of organic anion transporters (Figure 1A). . Sequence alignment and analysis of LST-2. (A) Amino acid alignment of LS...
Data files were searched against the gencode.v36.pc_translations protein database with sequences for mKO2 and mAG proteins manually added to the fasta file. The Sequest HT search algorithm was used, with precursor and fragment mass tolerances set to 10ppm and 0.6 Da, respectively. The ...
in darkness17,18,19,20,21. FourSPAgenes are present in the Arabidopsis genome, namelySPA1throughSPA4. All SPA proteins have a WD-repeat domain, a coiled-coil domain, and a kinase-like region22. Of these domains, the WD-repeat domain shows the highest sequence similarity among members23....