NIST reference materials (total protein standard, SRM 927e - Bovine Serum Albumin) and amino acids (SRM 2389a - Amino Acids) were used. The characterization of the test proteins is shown in the Electronic Supplementary Material: Protein Characterization. Peroxynitrite modification of high-purity BSA...
For method development, the bovine serum albumin solution (BSA) SRM 927e was obtained from NIST [24]. BSA is not the most suitable protein for the herein-developed method (due to high binding affinities to other compounds, possible restructuring of disulfide bonds, the reaction of free ...
In this contribution, the azo dye (E)-1-(4-((4-(phenylamino)phenyl)diazenyl) phenyl)ethanone (DPA) was combined with reduced graphene oxide (RGO) for the electrochemical modification of a pencil graphite electrode (RGO/DPA/PGE) surface. A series of electrochemical measurements were used for...
Increasing the X-ray diffraction power of protein crystals by dehydration: the case of bovine serum albumin and a survey of literature data Int. J. Mol. Sci., 13 (2012), pp. 3782-3800 View in ScopusGoogle Scholar [102] N.-J. Hu, S. Iwata, A.D. Cameron, D. Drew Crystal structure...
Liquid-phase acid hydrolysis of Bovine serum albumin (BSA). BSA standard (0.33 mL from a 2 mg/mL ampule, Thermo fisher scientific) was added to 0.67 mL of hydrolysis solution (as above) in a liquid-phase hydrolysis tube (resulting a final BSA concentration of 10 μM). Then 24 h hydrol...
8: 821-824 (1 974) Apoceruloplasmin copper deficiency holoceruloplasmin kinky hair syndrome neonate umbilical cord Wilson's disease Determination of Apoceruloplasmin by Radioimmunoassay in Nutritional Copper Deficiency, Menkes' Kinky Hair Syndrome, Wilson's Disease, and Umbilical Cord Blood Genetics Unit...
To the best of our knowledge, there is no report for application of portable HPLC in GSH determination. On the other hand, research on chromatography by microfluidics is progressing to allow separation with miniaturized columns through lab-on-a-chip technologies. Development is this area is highly...
Analyses of the pore size distribution in 3D matrices such as the cell-hydrogel interface are very useful when studying changes and modifications produced as a result of cellular growth and proliferation within the matrix, as pore size distribution plays
Wee SS, Ng YH, Ng SM (2013) Synthesis of fluorescent carbon dots via simple acid hydrolysis of bovine serum albumin and its potential as sensitive sensing probe for lead (II) ions. Talanta 116:71–76. doi:10.1016/j.talanta.2013.04.081 Article CAS Google Scholar Jana J, Ganguly M, Da...
We recorded activity of pronase e using different concentrations of bovine serum albumin (BSA) as substrate (Fig. 6e), which cannot be measured using other methods, and the initial rate data were obtained using IrCal (Fig. 6f). It should be mentioned that for pronase e the kinetic ...