Supplementary Figure 5 Protocol steps: bait, adenylated 3′ adapter and Bioanalyzer profiles. (a) Validation of RNA products post reverse transcription. Baits (Bait) were run in a 1% Agarose gel. RNase-treated baits (PCR temp) were used as a positive control, proving that the signal obtained...
Through the above four steps, one is connected to the DNA nucleotide on the solid carrier. Then the three chloroacetic acid deprotection of DMT group and its 5 - hydroxyl group, repeat the above steps until all the requirements of synthetic base have been grafted. The synthesis process can ...
The construction steps of MRP pseudo-sequence supertree was briefly illustrated in Fig. 1. Firstly, ten groups of CDSs for orthologous proteins in selected coronaviruses were organized using the OrthoMCL program27, with repeated sequences removed from the orthologous groups. The CDSs of 120 ...
Giardia intestinalis is an intestinal protozoan parasite that causes diarrheal infections worldwide. A key process to sustain its chain of transmission is the formation of infectious cysts in the encystation process. We combined deep RNAseq of a broad ra
Author(s):Lee J,Yoo E,Lee H,Park K,Hur JH,Lim C PubMed Article URL:http://dx.doi.org/10.1016/j.molcel.2017.03.004 R960-25 was used in Immunoprecipitation to demonstrate for the first time that ANKRD26 is expressed during the early steps of erythroid, megakaryocyte and granulocyte ...
(c) A lumped, schematic representation of the pathways in panel b (inside the dotted rectangle) showing also the higher steps of the cascade. Using these data, we show in Fig. 7, b and c, that the assembly proceeds via a few pathways that thread through the combinatorially complex ...
這不同密度的mitochondrial nucleoid-membrane complexes含有明顯不同量的粒線體膜系。在行體外mtDNA複製和轉錄結果中,我們確定不同粒線體的複製和轉錄的能力深受粒線體的膜系含量影響。我們因而確認粒線體基因的表達受粒線體DNA,粒線體核蛋白和粒線體膜系間的相關結構,組合關係密切。李寶林...
The maps are based on the crystal structure of recombinant HBc VLPs produced in bacteria9. Full size image Efficient column chromatography purification steps before alkaline treatment (see Supplementary Fig. S1 online) led to VLP preparations that demonstrated high consistency but different mobility by ...