Designed ankyrin repeat proteins (DARPins) have been developed into a robust and versatile scaffold for binding proteins. High-affinity binders are routinely selected by ribosome display and phage display. DARPins have entered clinical trials and have found numerous uses in research, due to their hi...
As a useful alternative Designed Ankyrin Repeat Proteins (DARPins) have been developed [7,8,9]. These designed proteins use the Ankyrin repeat fold consisting of two antiparallel helices, connected by a loop to the next unit. Three to five of these repeat units can be combined into a ...
Switzerland 1289 Designed ankyrin repeat proteins are a novel class of specific binding molecules, which display increased thermodynamic stability, smaller size and at least equal target affinity compared to immunoglobulins, making them potentially powerful tools in diagnostic pathology and therapeutic oncology...
By assembly of the randomized repeat modules, complex libraries of designed ankyrin repeat proteins (DARPins) can be generated. The number of randomized repeat modules used determines the size of the final protein, and with increasing numbers of repeat modules, the interaction interface and the ...
Designed to be stable: Crystal structure of a consensus ankyrin repeat protein — Supporting Figure 被引量: 0发表: 0年 High-affinity binders selected from designed ankyrin repeat protein libraries We report here the evolution of ankyrin repeat (AR) proteins in vitro for specific, high-affinity ta...
Computational-Modeling-of-Designed-Ankyrin-Repeat-Pro_2019_Journal-of-Molecu 上传人:文献在哪里·上传时间:2019-06-25 0% 0% 0% 0% 0% 继续阅读
Designed ankyrin repeat proteins (DARPins) that specifically bind to almost any target can be obtained by ribosome display or phage display from combinatorial libraries. Although DARPins are already very stable molecules, molecular dynamics simulations, equilibrium denaturation experiments, structural studies...
Breast cancer Designed ankyrin repeat proteins Human epidermal growth factor receptor 2 Molecular imaging Magnetic resonance imaging Superparamagnetic iron oxide 1. Introduction Breast cancer was reported to account for 30% of all new cancer diagnoses in women [1]. Human epidermal growth factor receptor...
Designed ankyrin repeat proteins (DARPins): binding proteins for research, diagnostics, and therapy. Annu Rev Pharmacol Toxicol 55, 489–511 (2015). 19. Zhou, H. X. & Bates, P. A. Modeling protein association mechanisms and kinetics. Curr Opin Struct Biol 23, 887–893 (2013). 20. ...
Binz et al., “Crystal Structure of a Consensus-Designed Ankyrin Repeat Protein: Implications for Stability,” PROTEINS: Structure, Function, and Bioinformatics (2006) 65:280-284. Binz et al., “Designed Repeat Proteins—Molecules with Antibody-like Binding Properties,” BIOforum Europe Apr. 200...