This online tool helps you to design primers and probes for your Real-time PCR (TaqMan) experiments. You can customize the potential PCR amplicon's size range, Tm (melting temperature) for the primers and probes, as well as the organism.
The PrimerQuest Tool helps to enable the design of basic and highly customized primers and probes for PCR and qPCR. Now that you understand the fundamentals of using this design tool, look for future articles from IDT about experiments requiring more complex assay design considerations, such as ...
Basic Standard Advanced ** This online tool designs PCR primers for you. Sequence Left primer Hybridization probe Right primer Reset FormPick Primers
For best results, PCR primer pairs should have closely matched melting temperatures to promote amplification of both strands in equal amounts. Use the Primer Characteristics dialog (Priming > Create Primer Pairs) to adjust the default target Tm. SeqBuilder Pro will attempt to locate primers as clo...
Primer Premier's search algorithm finds optimal PCR, multiplex and SNP genotyping primers with the most accurate melting temperature using the nearest neighbor thermodynamic algorithm. Primers are screened for secondary structures, dimers, hairpins, homologies and physical properties before reporting the ...
PCR (2 primers) qPCR (2 primers + probe; for use in 5' nuclease assays) qPCR (2 primers; for use with intercalataing dyes) Customize design parameters (optional) Select and order assays Click the box(es) next to the set(s) you wish to order. ...
If you are using the primers for cloning, we recommend cartridge purification as a minimum level of purification. If you are using the primers for mutagenesis, try to have the mismatched bases towards the middle of the primer. If you are using the primers fo...
Wojdacz TK, Borgbo T, Hansen LL (2009) Primer design versus PCR bias in methylation independent PCR amplifications . Epigenetics 4 : 231–234Wojdacz TK, Borgbo T, Hansen LL. Primer design versus PCR bias in methylation independent PCR amplifications. Epigenetics. 2009; 4 :231–234....
If you are using the primers for mutagenesis, try to have the mismatched bases towards the middle of the primer. If you are using the primers for a PCR reaction to be used in TOPO cloning, the primers should not have a phosphate modification. ...
Primer design:Inverse PCR (Circular Sequence) Minimal length (nt):Non-specific priming control Maximal length (nt):Overlaping primers Minimal Tm (°C):C>>T bisulfite conversion Maximal Tm (°C):qPCR MGB-probe design assay Minimal Linguistic Complexity (%):qPCR TaqMan-probe design assay ...