I would like to express my sincerest thanks to my thesis supervisor Dr. Maninder Singh, Assistant Professor, Computer Science and Engineering Department for his inspiration, guidance, stimulating suggestions, immense help and support throu...
We present GuideScan2 for memory-efficient, parallelizable construction of high-specificity CRISPR guide RNA (gRNA) databases and user-friendly design and analysis of individual gRNAs and gRNA libraries for targeting coding and non-coding regions in custom genomes. GuideScan2 analysis identifies widespre...
29,30], but none of them have addressed these issues thoroughly. In our study, we present a novel and powerful deep learning framework [31,32,33] to simultaneously predict sgRNA on-target knockout efficacy and a whole-genome off-target cleavage profile that competes favorably...
Gene editing has great therapeutic impact, being of interest for many scientists worldwide. Clustered regularly interspaced short palindromic repeats (CRISPR) technology has been adapted for gene editing to serve as an efficient, rapid, and cost-effective tool. To fulfill CRISPR experiment’s goals, ...
Using a powerful D3.js library enabled fast prototyping and development of the graphic component, as well as access to its advanced data visualization algorithms such as quadtree (https://github.com/d3/d3-quadtree) which is used in the “Force Layout” mode to detect collisions between data...
First, potential off-target sites are identified using user defined alignment algorithms (bowtie, bowtie2, blastn-short) [27–29] and summarized in the specificity score. Then, the annotation score evaluates the target position of sgRNAs within the respective gene model. Finally, the nucleotide...
Genome-wide knockout studies, noncoding deletion scans, and other large-scale studies require a simple and lightweight framework that can quickly discover and score thousands of candidate CRISPR guides targeting an arbitrary DNA sequence. While several C
Amrit K. BhullarDepartment of Electronics and Communication Engineering Punjabi University Patiala IndiaRanjit KaurDepartment of Electronics and Communication Engineering Punjabi University Patiala IndiaSwati SondhiDepartment of Electrical and Instrumentation Engineering Thapar University Patiala India...
Electrical and Instrumentation Engineering Department, Thapar Institute of Engineering and Technology, Patiala, IndiaShivam JainDepartment of Electrical Engineering, Indian Institute of Technology Roorkee, Roorkee, IndiaYogesh V. HoteDepartment of Electrical Engineering, Indian Institute of Technology Roorkee, ...
Keeping in view some discrepancy introduced due to non-linear response of the seat at different levels of vibration, the field results have been found to compare well with those obtained in the laboratory.Bansal, A.S.Thapar, S.INTER-NOISE and NOISE-CON Congress and Conference Proceedings...