This study purposed to describe the product Polymerase Chain Reaction (PCR) and sequencing of DNA Mycobacterium (M.) tuberculosis from sputum of tuberculosis (TB) patients in Medan. Sputum was collected from patients that diagnosed with pulmonary TB by a physician. Specimen processed by PCR method...
Background Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is the gold standard technique for mRNA quantification, but appropriate normalization is required to obtain reliable data. Normalization to accurately quantitated RNA has been proposed as the most reliable method for in vivo...
POLYMERASE CHAIN REACTIONHCV RNALIVERThe odd-mass Gd isotopes with mass number 153 A 157 are studied within the framework of the IBFA model. The single fermion is assumed to be in one of the 2 p 1 2, 2 p 3 2, 1 f 5 2, 1 f 7 2 or 0 h 9 2 single-particle orbitals. The ...
aMicroorganisms in respiratory tract of patients diagnosed with atypical pneumonia: results of a research based on the use of reverse transcription polymerase chain reaction (RT-PCR) DNA microarray method and enzyme-linked immunosorbent assay 微生物在患者呼吸道诊断以非典型肺炎: 根据对反向副本聚合酶链式...
polymerase chain reactionribosomal DNAThe pupal stage of Aedes pseudotaeniatus (Giles) is described for the first time. Collections of this species were made in the Himalayan Kingdom of Nepal. Group E of subgenus Finlaya is discussed.doi:10.1016/S0022-1910(97)00021-8Darsie RF...
(1971). Toward defining the course of evolution: Minimum change for a specific tree topology. Systematic Zoology, 20, 406–416. Article Google Scholar Giovannoni, S. J. (1991). The polymerase chain reaction. In E. Stackebrandt & M. Goodfellow (Eds.), Nucleic acid techniques in bacterial...
Changes of the rumen microbial community structure, as it can be established with a rumen simulation technique (RUSITEC) were studied using PCR and single-strand conformation polymorphism (SSCP) of small subunit rDNA genes (SSU rDNA). Four total mixed rations were incubated and two ammonia levels...
Polymerase chain reaction (PCR) products corresponding to the partial sequences of the mitochondrial cytochrome c oxidase subunit I (cox1) of eyeworms were Sanger sequenced and analyzed phylogenetically. Results A total of 118 (29.5%) camels from all five counties examined were infected with eye...
DNA preparations were used as templates in reaction mixtures containing a 0.5 μM concentration (each) of forward and reverse primers, 4 mM MgCl2, a 0.1 mM concentration of each deoxynucleoside triphosphate, 1 U of FastStart Taq DNA polymerase (Roche), and 10x reaction buffer (Roche). Cycling...
Study of β-Thalassemia Mutations Using the Polymerase Chain Reaction-Amplification Refractory Mutation System and Direct DNA Sequencing Techniques in a Gr... The aim of this study was the molecular characterization of β-thalassemia (thal) mutations in a group of 95 Egyptian thalassemic patients ...