Depurination of synthetic polynucleotide templates such as poly [d(A-T)] or poly [d(G-C)] leads to increased misincorporation of noncomplementary nucleotides when these templates are copied by prokaryotic and eukaryotic DNA polymerases. The ability of Escherichia coli DNA polymerase I to copy ...
Depurination by heat of DNA and DNA constituents, in solution and in the dry state, has been further investigated. The extent of depurination in solution varies inversely with the ionic strength and pH. Depurination at elevated temperatures in solution appears to be mainly an acid-catalysed hydroly...
The most critical step is the depurination of DNA molecules. The depurination by 0.2 M hydrochloric acid upon denaturation by sodium hydroxide leads to nicks in the DNA strands, resulting in a breakdown of long DNA fragments into shorter pieces of single-stranded DNA. These shorter fragments are...
The suppressing efficiencies of depurination of DNA by spermine, caldopentamine and caldohexamine in the presence of 1mM Mg 2+ , were approximately 50%, 60% and 80%, respectively. Mg 2+ also protected nucleic acids against depurination but to a lesser degree than polyamines. Longer unusual ...
Depurination has attracted considerable attention since a long time for it is closely related to the damage and repair of nucleic acids. In the present study, depurination using a pool of 30-nt short DNA pieces with various sequences at diverse pH values was analyzed by High Performance Liquid...
Depurinated bases in single-stranded DNA undergoing replication can lead to mutations, because in the absence of information from the complementary strand, BER can add an incorrect base at the apurinic site, resulting in either a transition or transversion mutation. WikiMatrix More complex compoun...
Base excision repair of DNA in eukaryotes involves endonucleolytic cleavage of the sugar-phosphate backbone upstream of the missing base, insertion of an intact nucleotide and removal of the sugar-phosphate fragment by polymeraseβ, followed by ligation of the DNA (40, 41). Although the process ...
Nearly 80% of benzo[a]pyrene (BP)-DNA adducts are lost by depurination and excreted in the urine. These adducts offer a unique opportunity for biomonitoring BP exposure, although their hydrophobic nature poses challenges for methods development. We have implemented a competitive enzyme-linked ...
Depurination of synthetic polynucleotide templates such as poly[d(A-T))] or poly[d(G-C)] leads to increased misincorporation of noncomplementary nucleotides when these templates are copied by prokaryotic and eukaryotic DNA polymerases.The ability of Escherichia coli DNA polymerase I to copy over ...
International Journal of Mass Spectrometry & Ion ProcessesGut IG. Depurination of DNA and matrix-assisted laser desorption/ionization mass spectrometry. Int. J. Mass Spectrom. Ion Proc. 1997; 169-170 :313–322.Gut,I.G. (1997) Depurination of DNA and matrix-assisted laser desorption/ionization ...